米诺环素对创伤后应激障碍大鼠神经炎症的影响
The effects of minocycline on neuroinflammation in rats with post-traumatic stress disorder
张冲 1石佐林 1王振 1董玉书1
作者信息
- 1. 中国人民解放军北部战区总医院神经外科,沈阳 110016
- 折叠
摘要
目的:研究米诺环素对创伤后应激障碍(PTSD)大鼠神经炎症反应的影响.方法:采用单次延长应激(SPS)方法制备大鼠PTSD模型,通过灌胃方法给予大鼠米诺环素治疗(PTSD+Mino group)或者生理盐水(PTSD group).通过黑白箱穿梭实验检测大鼠的行为变化,免疫组织化学染色检测海马部位离子钙结合适配分子1(Iba-1)的表达,ELISA检测脑海马IL-1β和TNF-α的含量,实时定量RT-PCR(qRT-PCR)方法检测海马部位IL-1β和TNF-α mRNA的表达水平.结果:SPS刺激后大鼠3 d后,大鼠焦虑样行为明显,海马Iba-1表达增加,海马组织中IL-1β和TNF-α含量增加.而米诺环素治疗明显减少PTSD大鼠焦虑样行为,降低海马中Iba-1的表达,同时米诺环素治疗还可以降低海马IL-1β和TNF-α mRNA和蛋白的水平.结论:米诺环素治疗可以通过抑制小胶质细胞活化改善PTSD大鼠的焦虑样行为.
Abstract
Objective:To investigate the effect of minocycline on neuroinflammation of rats with post-traumatic stress disorder(PTSD).Methods:The rat model of PTSD was prepared by a single prolonged stress(SPS)method,and the rats were treated with minocycline(PTSD+Mino group)or normal saline(PTSD group)by gavage.The behavioral changes of rats were detected by light-dark box test.The expression of ionized calcium-binding adapter molecule 1(Iba-1)in hippocampus was detected by immunohistochemical staining.The contents of IL-1β and TNF-α in hippocampus were detected by ELISA,and the expression levels of IL-1β and TNF-α mRNA in hippocampus were detected by real-time RT-PCR(qRT-PCR).Results:After 3 days of SPS stimulation,the anxiety-like behavior of rats was obvious,the expression of Iba-1 in hippocampus was increased,and the contents of IL-1β and TNF-α in hippocampus were in-creased.Minocycline treatment significantly reduced anxiety-like behavior and decreased the expression of Iba-1 in the hippocampus of PTSD rats.Meanwhile,minocycline treatment also decreased the levels of IL-1β and TNF-α mRNA and protein in the hippocampus.Conclusion:Minocycline can improve the anxiety-like behavior of PTSD rats by inhibiting the activation of microglia.
关键词
米诺环素/创伤后应激障碍/神经炎症/小胶质细胞/海马/大鼠Key words
minocycline/post-traumatic stress disorder(PTSD)/neuroinflammation/microglia/hippocampus/rat引用本文复制引用
出版年
2024
NETL
NSTL
万方数据