舒血宁注射液通过Wnt/β-catenin信号通路保护大鼠缺血性脑损伤
Shuxuening injection protects rats from cerebral ischemia through the Wnt/β-catenin signaling pathway
谢明 1陈早根 1鞠蔚华2
作者信息
- 1. 南昌大学抚州医学院人体解剖学教研室,抚州 344000
- 2. 南昌市第九医院感染一科,南昌 330000
- 折叠
摘要
目的:研究舒血宁(SXN)对缺血性脑损伤大鼠海马Wnt/β-catenin信号通路的影响.方法:SD大鼠分为3组:假手术(sham)组、大脑中动脉栓塞(MCAO)组和MCAO手术处理+SXN治疗(MCAO+SXN)组.采用MCAO法制备大鼠缺血性脑损伤模型,SXN组大鼠通过尾静脉注射SXN.采用神经功能损伤评分和平衡木试验检测大鼠神经功能,利用HE染色观察海马CA1区炎症细胞浸润的变化,利用免疫荧光染色观察海马CA1区β-catenin表达,通过real time RT-PCR和Western Blot方法分别检测海马区胱天蛋白酶3(caspase-3)、环氧合酶2(COX-2)和内皮型一氧化氮合成酶(eNOS)mRNA和蛋白的表达.结果:SXN能改善脑缺血再灌注大鼠神经功能障碍,减少海马CA1区炎性细胞浸润,并降低β-catenin、caspase-3和COX-2的表达,同时上调eNOS表达.结论:舒血宁通过抑制Wnt/β-catenin信号通路对大鼠炎症反应、氧化应激及神经细胞凋亡保护缺血性脑损伤.
Abstract
Objective:To study the effects of Shuxuening injection(SXN)on Wnt/β-catenin signaling pathway in hippocampus of rats with cerebral ischemia.Methods:SD rats were divided into three groups:Sham operation group(Sham),middle cerebral artery occlusion group(MCAO)and MCAO+SXN treatment group(MCAO+SXN).The model of cerebral ischemia in rats was prepared by MCAO.The rats with cerebral ischemia were treated with SXN by caudal vein injection.Zea-Longa scoring criteria and balance beam test were employed to evaluated neurological function of rats.HE staining were used to observe the changes of inflammatory cells infiltration the hippocampal CAI region.The expression of β-catenin in hippocampal CA1 region was observed by immunofluorescence staining.The mRNA and pro-tein expressions of caspase-3,cyclooxygenase-2(COX-2)and endothelial nitric oxide synthetase(eNOS)in hippocam-pus were detected by real time RT-PCR and Western Blot,respectively.Results:SXN can SXN can improve the neuro-logical dysfunction of cerebral ischemia rats.The inflammatory cells infiltration in hippocampal CAI region was decreased,and the expression of β-catenin,caspase-3 and COX-2 was decreased,while the expression of eNOS was upregulated.Conclusion:SXN protects against cerebral ischemia by inhibiting Wnt/β-catenin signaling pathway against inflammation response,oxidative stress and apoptosis of nerve cells in rats.
关键词
Wnt/β-catenin信号通路/舒血宁/缺血性脑损伤/海马/大鼠Key words
Wnt/β-catenin signaling pathway/Shuxuening injection(SXN)/cerebral ischemia/hippocampus/rat引用本文复制引用
基金项目
江西省教育厅科学技术研究青年项目(GJJ218115)
南昌大学抚州医学院科技一般项目(fykj202205)
南昌大学抚州医学院科技一般项目(fykj202208)
出版年
2024
NETL
NSTL
万方数据