Objective:To explore the central regulatory mechanisms of exercise to alleviate the behavioral effects of anxiety and depression in post-traumatic stress disorder(PTSD)and to promote hippocampal neurogenesis.Methods:Male C57BL/6J mice were randomly divided into control group(Control),PTSD modeling group(PTSD),PTSD-mod-eling with low-intensity exercise group(PTSD+LE)and PTSD-modeling with high-intensity exercise group(PTSD+HE).A combination of conditioned foot shock(CF)and single-sustained stress(SPS)was used to construct the PTSD compound stress model.The anxiety and depression-like behaviors of mice were assessed using the open field test and the tail suspension test,respectively.Newborn mature neurons and proliferating cells in the DG area of mice hippocam-pus were observed by immunofluorescence double-labeling experiment.The expression of mice hippocampal adiponectin receptor 1(AdipoR1)was detected by Western Blot.Results:The results of the open field test showed that the mice in the PTSD+HE group showed a significantly longer distance and stay in the central area than those in the PTSD group and the PTSD+LE group(P＜0.05);the results of the tail suspension test showed that the immobility time of the mice in the groups with different levels of locomotor training was significantly reduced compared with that of the mice in the PTSD group(P＜0.05),and it was more significant in the PTSD+HE group(P＜0.05).In addition,BrdU+,Br-dU+/NeuN+and MCM2+cell densities in the hippocampal DG region of mice in the PTSD+HE group were significant-ly higher(P＜0.05).And the hippocampal AdipoR1 protein expression of mice in the PTSD+HE group was signifi-cantly upregulated(P＜0.05).Conclusion:Anxiety and depression-like behaviors in PTSD mice are associated with decreased levels of hippocampal neurogenesis.Exercise can improve their anxiety and depression-like behaviors,and the mechanism of the effect may be related to the promotion of AdipoR1 expression and hippocampal neurogenesis.
维普
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