小鼠丘脑背内侧核内VGLUT2阳性神经元传入联系的形态学研究
Morphological study of afferent connections of VGLUT2 positive neurons of mediodorsal thalamic nucleus in mice
冯慧洁 1彭霏 2薛美琪 1阮彩莲 3董玉琳4
作者信息
- 1. 空军军医大学基础医学院人体解剖与组织胚胎学教研室暨梁銶琚脑研究中心,西安 710032;延安大学基础医学院人体解剖学教研室,延安 716000
- 2. 空军军医大学基础医学院学员五大队,西安 710032
- 3. 延安大学基础医学院人体解剖学教研室,延安 716000
- 4. 空军军医大学基础医学院人体解剖与组织胚胎学教研室暨梁銶琚脑研究中心,西安 710032
- 折叠
摘要
目的:利用狂犬病病毒(RV)介导的逆行跨单级突触追踪技术,观察丘脑背内侧核(MD)内囊泡膜谷氨酸转运体2(VGLUT2)阳性神经元的全脑突触前神经元分布.方法:将RV的辅助病毒注射至VGLUT2-ires-Cre转基因小鼠右侧MD,两周后将RV注入相同区域.一周后灌注取材,进行全脑扫描,观察RV标记的突触前神经元在全脑的分布.结果:将RV病毒注入VGLUT2-ires-Cre小鼠MD后,在皮质和脑干内可观察到密集的突触前神经元.皮质内逆标神经元多分布于运动皮质、内侧前额叶皮质、眶额皮质和岛叶;丘脑内多见于丘脑网状核和下丘脑外侧区等;而脑干逆标神经元则主要分布在臂旁外侧核、中脑导水管周围灰质和中缝核等部位.结论:MD内VGLUT2阳性神经元一方面可接受来自脑干的上行纤维的投射,或丘脑网状核的信息调控;另一方面作为高阶丘脑也可接受皮质的下行投射,参与脑内的多种功能.以上结果为研究MD的功能及相关神经环路的机制提供了形态学基础.
Abstract
Objective:Using retrogradely trans-monosynaptic tracing method mediated by rabies virus(RV)to ob-serve presynaptic neuronal distributions of vesicular glutamate transporter 2(VGLUT2)-positive neurons of the med-iodorsal thalamic nucleus(MD)in whole brain.Methods:The helper viruses of RV were injected into the right MD of VGLUT2-ires-Cre mice,and two weeks later RV was injected into the same area.After another one week,perfusion was taken and whole brain scanning was performed to observe the distributions of RV-labeled presynaptic neurons throughout the brain.Results:After RV virus was injected into the MD of VGLUT2-ires-Cre mice,dense presynaptic neurons were observed in the cortex and brainstem.RV labeled neurons were mostly distributed in primary motor cortex(M1),sec-ondary motor cortex(M2),medial prefrontal cortex,orbitofrontal cortex and insular cortex at the cortical level.Tha-lamic retrogradely labeled neurons were mostly found in reticular thalamic nucleus and lateral hypothalamic area,and retrogradely labeled neurons in the brainstem were mainly distributed in lateral parabrachial nucleus,periaqueductal grey matter and dorsal raphe nucleus.Conclusion:The results in the present experiment suggest that VGLUT2-positive neurons within the MD can receive ascending information transmission from the brainstem,or regulation from the reticu-lar nucleus of thalamus.As a higher-order thalamus,MD can also receive descending projections from the cortex,and is involved in a variety of functions.Our results provide morphological basis for the study of the function and the related neural circuit of the MD.
关键词
丘脑背内侧核/狂犬病病毒/囊泡膜谷氨酸转运体2/小鼠Key words
mediodorsal thalamic nucleus(MD)/rabies virus(RV)/vesicular glutamate transporters 2(VGLUT2)/mouse引用本文复制引用
基金项目
国家自然科学基金(32271045)
陕西省重点研发计划一般项目(2023-YBSF-156)
出版年
2024
NETL
NSTL
万方数据