通过自制套管给予小脑延髓池脂多糖诱发大鼠中枢神经系统感染
Lipopolysaccharide was administered via a self-made cannula in the cerebellomedullary cistern of rats to induce CNS infection
郭瑶 1郭畅 1王凯妮 2臧若琪 2高洁 2马洋 2陈怡彤 2张宇3
作者信息
- 1. 山西医科大学口腔医学院,太原 030001
- 2. 山西医科大学生理学系暨细胞生理学教育部重点实验室,太原 030001
- 3. 山西医科大学口腔医学院,太原 030001;山西医科大学生理学系暨细胞生理学教育部重点实验室,太原 030001
- 折叠
摘要
目的:本研究旨在应用自制给药套管植入大鼠小脑延髓池(CMC),以实现给清醒大鼠多次给药.方法:自制给药套管,从大鼠颅骨枕外嵴中点开孔,沿枕骨内壁下行直至套管尖端达CMC,然后颅钉和自凝树脂固定.应用脂多糖(LPS)作为局部炎性刺激物注射到CMC诱发神经炎症,并使用X线、行为学检测及免疫荧光染色评估该方法的可行性.结果:X线及滂胺天蓝染料确认脑内置管埋置位置合适.经给药管注射LPS组大鼠较Control组大鼠面部机械痛阈值降低(P＜0.001);LPS组大鼠在旷场中央停留时间较Control组大鼠显著下降(P＜0.01).免疫荧光染色结果表明LPS激活三叉神经脊束核尾侧亚核(SpVc)小胶质细胞.结论:该方法可有效作用于大鼠SpVc,适用于清醒大鼠小脑延髓池内的重复多次给药.
Abstract
Objective:This study introduces a novel approach utilizing a self-made drug delivery cannula implanted into the cerebellomedullary cistern(CMC)of rats to allow repeated administrations in conscious subjects.Methods:A self-made medication cannula is inserted through a drilled hole at the midpoint of the occipital crest of the rat's skull,de-scending along the inner wall of the occipital bone until reaching the CMC,and securing it in place with skull screws and self-curing resin.Lipopolysaccharide(LPS)is injected into the CMC to induce neuroinflammation,and the feasibility of this method is assessed using X-ray imaging,behavioral testing,and immunofluorescence staining.Results:The place-ment of the brain cannula was confirmed using X-ray film and pontamine sky blue staining.Rats in the LPS group exhib-ited a lower facial mechanical pain threshold compared to the Control group(P＜0.001),along with reduced residence time in the open field center(P＜0.01).Immunofluorescence staining revealed LPS-induced activation of caudal spinal trigeminal nucleus(SpVc)microglia.Conclusion:This method proves to be suitable for multiple administrations to the cerebellomedullary cistern of conscious rats,enabling the study of the SpVc's role in pain modulation.
关键词
三叉神经脊束核尾侧亚核/小脑延髓池/口面部疼痛/置管给药/神经炎症/大鼠Key words
cerebellomedullary cistern(CMC)/caudal spinal trigeminal nucleus(SpVc)/oralfacial pain/catheter administration/neuroinflammation/rat引用本文复制引用
出版年
2024
NETL
NSTL
万方数据