星状神经节阻滞对大鼠炎症反应的作用及其机制研究
The effects of stellate ganglion block on inflammatory response in rats and its mechanism
夏雪 1杨鹏 1龙方方 1覃禹翱 1李林 1邹学军1
作者信息
- 1. 三峡大学附属仁和医院麻醉科,宜昌 443001
- 折叠
摘要
目的:观察星状神经节阻滞(SGB)对急性腹膜炎大鼠血清炎症因子的影响和α7nAChR蛋白表达情况,旨在深入探讨SGB对大鼠急性腹膜炎炎症反应的作用机制.方法:40只SPF级雄性大鼠随机分为4组:对照组(Control)、急性腹膜炎组(AP)、急性腹膜炎+星状神经节阻滞组(AP+SGB)、急性腹膜炎+星状神经节阻滞+α7nAChR抑制剂甲基牛扁亭碱组(AP+SGB+MLA),每组10只.将2％乙酸1 ml/100 g分别注入AP组、AP+SGB组和AP+SGB+MLA组大鼠腹腔内建立急性腹膜炎模型.ELISA法检测血清中IL-18和TNF-α的浓度.Western Blot法检测腹膜组织中α7nAChR蛋白水平,RT-qPCR法检测腹膜组织中α7nAChR mRNA的表达情况.结果:与Control组对比,其余3组血清IL-18和TNF-α水平增高(P＜0.05);与AP组对比,AP+SGB组血清IL-18和TNF-α水平降低(P＜0.05);与AP+SGB组对比,AP+SGB+MLA组血清IL-18和TNF-α水平增高(P＜0.05).腹膜组织α7nAChR蛋白和α7nAChR mRNA表达水平的比较:与Control组对比,其余3组α7nAChR蛋白和α7nAChR mRNA表达水平均增高(P＜0.05);与AP组对比,AP+SGB组o7nAChR蛋白和α7nAChR mRNA 表达水平增高(P＜0.05);与 AP+SGB 组相比,AP+SGB+MLA 组 α7nAChR 蛋白和 α7nAChR mRNA表达水平降低(P＜0.05).结论:星状神经节阻滞治疗可以减少大鼠炎症因子的产生,抑制急性腹膜炎的炎症反应,其抗炎机制可能与α7nAChR介导的胆碱能抗炎通路(CAP)有关.
Abstract
Objective:To observe the effect of stellate ganglion block(SGB)on serum inflammatory factors and the expression of α7nAChR protein in rats with acute peritonitis,so as to further explore the mechanism of SGB on the inflammatory response of acute peritonitis in rats.Methods:40 SPF grade male rats were randomly divided into 4 groups:blank group(Control),acute peritonitis group(AP),acute peritonitis+stellate ganglion block group(AP+SGB),acute peritonitis+stellate ganglion block+α7nAChR inhibitor methyllycaconitine group(AP+SGB+MLA),with 10 rats in each group.Inject 2％acetic acid at a dose of 1 ml/100 g into the peritoneal cavity of rats to establish an acute peritonitis model in the AP group,AP+SGB group,and AP+SGB+MLA group.Blood samples were collected from the tail vein of the rats,and the concentrations of IL-18 and TNF-α in serum were detected by ELISA.Western Blot method was used to detect the level of α7nAChR protein in the peritoneal tissues,and RT-qPCR method was used to detect the expression of α7nAChR mRNA in the peritoneal tissues.Results:Compared with Control group,the levels of IL-18 and TNF-α were increased in the other three groups(P＜0.05).Compared with AP group,serum levels of IL-18 and TNF-α in AP+SGB group were decreased(P＜0.05).Compared with AP+SGB group,serum levels of IL-18 and TNF-α were increased in AP+SGB+MLA group(P＜0.05).The expression of α7nAChR mRNA protein were higher in the three groups than group Control,(P＜0.05).Compared with AP group,α7nAChR protein and α7nAChR mRNA expression levels were higher in AP+SGB group and AP+SGB+MLA group(P＜0.05).Compared with AP+SGB group,α7nAChR protein and α7nAChR mRNA expression levels in AP+SGB+MLA group were lower(P＜0.05).Conclusion:Stellate ganglion block treatment can reduce the production of inflammatory factors and inhibit the inflammatory response of acute peritonitis in rats,and its anti-inflammatory mechanism may be related to the cholinergic anti-inflammatory pathway(CAP)mediated by α7nAChR.
关键词
星状神经节阻滞/急性腹膜炎/炎症因子/胆碱能抗炎通路/大鼠Key words
stellate ganglion block(SGB)/acute peritonitis(AP)/inflammatory factors/cholinergic anti-inflam-matory pathway(CAP)/rat引用本文复制引用
基金项目
湖北省自然科学基金(2023AFC021)
出版年
2024
NETL
NSTL
万方数据