Src激酶抑制剂PP2对慢性酒精暴露大鼠学习记忆能力的影响
The effects of Src kinase inhibitor PP2 on learning and memory in rats with chronic alcohol exposure
李志达 1张勇2
作者信息
- 1. 鹤壁职业技术学院医学院医学影像技术教研室,鹤壁 458030
- 2. 空军军医大学基础医学院人体解剖与组织胚胎学教研室,西安 710032
- 折叠
摘要
目的:研究Src激酶抑制剂PP2对慢性酒精暴露大鼠学习记忆能力的影响及分子机制.方法:通过饮用方法制备大鼠慢性酒精暴露模型,通过腹膜腔注射给予PP2.Morris水迷宫检测大鼠学习记忆能力,通过酶联免疫吸附试验(ELISA)检测大鼠海马中IL-1β和TNF-α的含量,通过Western Blot检测大脑海马Src、Iba-1和iNOS的表达.结果:慢性酒精暴露可导致大鼠逃避潜伏期延长,逃避时间延长.同时海马中Src、Iba-1和iN-OS的表达增加,以及IL-1β和TNF-α的含量增加.PP2能够降低慢性酒精暴露大鼠的逃避潜伏期,降低海马中Src、Iba-1和iNOS表达.此外,IL-iβ和TNF-α的含量下降.结论:PP2通过抑制神经系统炎症改善慢性酒精暴露导致学习记忆能力障碍.
Abstract
Objective:To study the effect of Src kinase inhibitor PP2 on learning and memory in rats with chronic alcohol(EtOH)exposure and its molecular mechanism.Methods:The chronic alcohol exposure model of rats was pre-pared by drinking method,and PP2 was given by intraperitoneal injection.The learning and memory ability of rats was detected by Morris water maze,the contents of IL-1β and TNF-α in hippocampus were detected by enzyme linked im-munosorbent assay(ELISA),and the expressions of Src,Iba-1 and iNOS in hippocampus were detected by Western Blot.Results:Chronic alcohol exposure could prolong the incubation period and escape time of rats.At the same time,the expression of Src,Iba-1 and iNOS,as well as the contents of IL-1β and TNF-α were increased in the hippocampus.PP2 can decrease the escape latency and the expression of Src,Iba-1,and iNOS in hippocampus of chronic alcohol exposed rats.In addition,the levels of IL-1β and TNF-α decreased.Conclusion:PP2 improves learning and memory impairment caused by chronic alcohol exposure by inhibiting nervous system inflammation.
关键词
慢性酒精暴露/学习记忆/Src激酶抑制剂/神经炎症/大鼠Key words
chronic alcohol exposure/learning and memory/Src kinase inhibitor/neuroinflammation/rat引用本文复制引用
出版年
2024
NETL
NSTL
万方数据