骨桥蛋白抑制蛛网膜下腔出血大鼠的小胶质细胞向M1极化
Osteopontin inhibits M1 polarization of microglia in rats with subarachnoid hemorrhage
陈立刚 1邹正 1郝广志 1董玉书1
作者信息
- 1. 中国人民解放军北部战区总医院神经外科,沈阳 110016
- 折叠
摘要
目的:骨桥蛋白(OPN)在多种脑卒中模型中显示出神经保护作用.它在脑损伤后神经炎症中的作用仍有待阐明.本研究旨在阐明OPN对蛛网膜下腔出血(SAH)后小胶质细胞功能状态的影响.方法:30只大鼠随机分为Sham组、SAH组和SAH+OPN组,利用二次注射自体动脉血方法制备SAH大鼠模型,治疗组通过鼻饲法给予OPN.利用改良Garcia评分法评估神经功能,通过检测脑含水量评估大鼠脑水肿的程度,RT-qPCR检测小胶质细胞激活状态标志物CD86、诱导型一氧化氮合酶(iNOS)、CD206和精氨酸酶1(Arg-1)在SAH和OPN处理后的表达.酶联免疫吸附试验(ELISA)方法检测脑脊液中IL-1β、IL-6、IL-10和IL-13的含量.结果:经鼻给药OPN可改善SAH大鼠神经功能障碍和脑水肿,抑制脑内CD86、iNOS、1L-1β、IL-6的表达,并促进CD206、Arg-1、IL-10和IL-13的表达.结论:OPN通过抑制小胶质细胞M1极化减轻SAH后的炎症反应.
Abstract
Objective:Osteopontin(OPN)has demonstrated neuroprotective effects in various stroke models.Its role in neuroinflammation after brain injury remains to be elucidated.This study aims to clarify the effect of OPN on neuroin-flammation,particularly on the functional states of microglia after subarachnoid hemorrhage(SAH).Methods:Thirty rats were randomly divided into the following groups:Sham,SAH,and SAH+OPN.SAH rat model was prepared by secondary injection of autologous arterial blood,and OPN was given intranasally in the treatment group.Neurological function was evaluated by modified Garcia score.The degree of cerebral edema was evaluated by measuring brain water content.The expression of microglia activation markers CD86,inducable nitric oxide synthase(iNOS),CD206 and arginase 1(Arg-1)after SAH and OPN treatment was detected by RT-qPCR.The levels of IL-1β,IL-6,IL-10,and IL-13 in cerebrospinal fluid were detected by enzyme-linked immunosorbent assay(ELISA).Results:Intranasal ad-ministration of OPN could improve the neurological dysfunction and cerebral edema in SAH rats.What's more,OPN could inhibit the expression of CD86,iNOS,IL-1β,and IL-6 in cerebral while promote the expression of CD206,Arg-1,IL-10,and IL-13.Conclusion:OPN alleviates the inflammatory response after SAH by inhibiting the polarization of microglia M1.
关键词
骨桥蛋白/蛛网膜下腔出血/早期脑损伤/小胶质细胞/大鼠Key words
osteopontin(OPN)/subarachnoid hemorrhage(SAH)/early brain injury(EBI)/microglia/rat引用本文复制引用
出版年
2024
NETL
NSTL
万方数据