Objective:To improve the operability and stability of the middle cerebral artery occlusion(MCAO)model,we established a novel method,external carotid artery ligation free(ECA-LF),for MCAO model and compared its advantages with the traditional Longa method and Koizumi method.Methods:A total of 100 male C57BL/6 mice were randomly divided into four groups:sham group,Koizumi group,Longa group,and ECA-LF group.The advantages and disadvantages of each method were evaluated in terms of surgical operation time,postoperative animal survival rate,postoperative animal survival rate,neurological functional score,and stability of infarct size.Results:The ECA-LF method spent less time than either the Longa or the Koizumi method during the course of making MCAO model.Com-pared with the Longa group,the ECA-LF group had a lower Garcia score,but was comparable to the Koizumi group.The survival rate of animals in the ECA-LF group was significantly higher than the other two groups 7 days after surgery.The Morris water maze(MWM)test showed that there was no significant difference between the ECA-LF group and oth-er two groups in learning and memory ability.The degree of cerebral edema and infarct size in the ECA-LF group were comparable to the other two groups.Conclusion:Compared with the traditional Longa and Koizumi methods,the ECA-LF method simplifies the surgical procedures,shortens the model-making time,and improves the survival rate of model animal with similar stability to the traditional methods.It has certain advantages in stroke experimental research.
维普
万方数据