Objective:To study the repair impact of recombinant human collagen Ⅲ(rhCol Ⅲ)hydrogel with sus-tained cell-free fat extract(CEFFE)release on spinal cord injury(SCI).Methods:The rhCol Ⅲ hydrogel was com-bined with CEFFE to create a composite scaffold capable of releasing CEFFE.Rats with SCI hemicut models were divid-ed into three groups:SCI,rhCol Ⅲ,and rhCol Ⅲ/CEFFE.Results:The rhCol Ⅲ/CEFFE hydrogel features a three-dimensional(3D)porous structure and suitable elasticity,enabling continuous CEFFE release.This group demonstra-ted notable motor function improvement and more complete spinal cord tissue continuity compared to other greups.The enzyme-linked immunosorbent assay(ELISA),Luxol fast blue staining(LFB),and immunofluorescence results dem-onstrated that RhCol Ⅲ/CEFFE hydrogel inhibits inflammatory factors,encourages microglia M2 transformation,reduces glial scar formation,and promotes neuronal differentiation,axonal regeneration,and myelin sheath formation.Conclusion:The rhCol Ⅲ/CEFFE hydrogel reshapes the inhibitory microenvironment,supports nerve regeneration and neural network formation,and aids in neural function recovery.
维普
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