Objective:To demonstrate the three-dimensional structures of the limbic system and its fiber connections through fiber dissection,and to provide reference for relevant professionals to master the anatomy of the limbic system.Methods:Ten cerebral hemispheres were treated and dissected according to Kelinger method,and the limbic system and its fiber connections were displayed.Results:The limbic system was arranged around the thalamus and corpus cal-losum in a double-layer concentric circle structure.The outer layer structures mainly consisted of the cingulate gyrus and the parahippocampal gyrus,while the inner layer structures included the amygdala,hippocampus and fornix.The main association fiber of the outer layer is the cingulum,whose superior trunk is mainly located in the cingulate gyrus,and the inferior trunk is mainly located in the parahippocampal gyrus.The fiber structures of the inner layer includes the striae terminalis and ansa peduncularis emanating from the amygdala and the fornix of the hippocampus.Conclusion:Limbic system is an important connection structure between telencephalon and diencephalon,and its anatomical struc-ture is complex.Fiber dissection method can effectively demonstrate the complex spatial structure of limbic system,which is of great benefit to relevant professionals to understand its three-dimensional structure.