神经解剖学杂志2024，Vol.40Issue(5) ：607-612.DOI:10.16557/j.cnki.1000-7547.2024.05.011

枸杞多糖通过SLC7A11/GPX4通路抑制铁死亡减轻小鼠脑缺血再灌注损伤

Lycium barbarum polysaccharide inhibits iron death through SLC7A11/GPX4 pathway to reduce cerebral ischemia-reperfusion injury in mice

邱政皓 马雅萍 马昌盛 韩博 孟姝辰 白敏 贺茂涛
神经解剖学杂志2024，Vol.40Issue(5) ：607-612.DOI:10.16557/j.cnki.1000-7547.2024.05.011
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枸杞多糖通过SLC7A11/GPX4通路抑制铁死亡减轻小鼠脑缺血再灌注损伤

Lycium barbarum polysaccharide inhibits iron death through SLC7A11/GPX4 pathway to reduce cerebral ischemia-reperfusion injury in mice

邱政皓 1马雅萍 1马昌盛 1韩博 1孟姝辰 1白敏 1贺茂涛2
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作者信息

  • 1. 山东第二医科大学:基础医学院,潍坊 261021
  • 2. 山东第二医科大学:基础医学院,潍坊 261021;山东第二医科大学:附属医院,潍坊 261021
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摘要

目的:探讨枸杞多糖(LBP)是否通过溶质载体家族7成员11(SLC7A11)/谷胱甘肽过氧化酶4(GPX4)通路抑制铁死亡进而减轻脑缺血再灌注损伤.方法:使用大脑中动脉栓塞(MCAO)方法建立小鼠脑缺血再灌注(I/R)损伤模型,并同时给予枸杞多糖处理.小鼠被随机分为3组:假手术(Sham)组,I/R组,以及I/R+LBP组.使用2,3,5氯化三苯基四氮唑(TTC)染色来观察脑梗死体积;通过神经功能缺损评分来评估小鼠的神经功能;利用超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、丙二醛(MDA)和亚铁离子(Fe2+)检测试剂盒来检测SOD、GSH、MDA和Fe2+的水平变化;利用Western Blot检测抗铁死亡相关蛋白,包括SLC7A11、GPX4以及核因子红细胞系2相关因子2(Nrf2)的表达情况.结果:枸杞多糖能够减少I/R小鼠的脑梗死体积,改善I/R小鼠神经功能;枸杞多糖增加I/R损伤小鼠中SOD和GSH的含量、减少MDA的含量;Western Blot结果:I/R组脑组织中抗铁死亡相关蛋白SLC7A11、GPX4、Nrf2的表达水平明显低于Sham组;而通过枸杞多糖处理后,I/R+LBP组中SLC7A11、GPX4、Nrf2的水平明显高于I/R组.结论:枸杞多糖可能通过SLC7A11/GPX4信号通路抑制铁死亡来减轻脑缺血再灌注损伤.枸杞多糖对脑缺血再灌注损伤具有神经保护作用,是一种潜在的神经保护剂.

Abstract

Objective:To investigate whether Lycium barbarum polysaccharides(LBP)can inhibit ferroptosis through the solute carrier family 7 member 11(SLC7A11)/glutathione peroxidase 4(GPX4)pathway and thus alleviate cerebral ischemia-reperfusion(I/R)injury.Methods:The mouse model of cerebral I/R injury was established by mid-dle cerebral artery occlusion(MCAO)and treated with Lycium barbarum polysaccharides(LBP).The mice were ran-domly divided into three groups:sham group,I/R group,and I/R+LBP group.2,3,5triphenyltetrazolium chloride(TTC)staining was used to observe the volume of cerebral infarcts;Neurological function was assessed by neurological deficit score;The superoxide dismutase(SOD),glutathione(GSH),malondialdehyde(MDA),and Fe2+detection kit to detect the changing levels of SOD,GSH,MDA,and Fe2+;Western Blot detects the expression of ferroptosis-related keys GPX4,SLC7A11,and Nrf2.Results:LBP can reduce the cerebral infarction volume and improve the neurological function.LBP increases the content of SOD and GSH and reduces the content of MDA in I/R injured mice.Western Blot results show that the expression levels of ferroptosis-related proteins GPX4,SLC7A11,and Nrf2 in the brain tissue of the I/R group were significantly lower than those of the Sham group.After LBP treatment,the levels of GPX4,SLC7A11,and Nrf2 in the LBP+I/R group were significantly higher than those of the I/R group.Conclusion:LBP at-tenuates cerebral I/R injury by inhibiting ferroptosis through the SLC7A11/GPX4 pathway.LBP has a neuroprotective effect on cerebral I/R injury and is a potential neuroprotective agent.

关键词

枸杞多糖/缺血再灌注损伤/SLC7A11/GPX4/铁死亡/小鼠

Key words

Lycium barbarum polysaccharide(LBP)/ischemia-reperfusion(I/R)/solute carrier family 7 member 11(SLC7A11)/glutathione peroxidase 4(GPX4)/ferroptosis/mouse

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出版年

2024
神经解剖学杂志
中国解剖学会，第四军医大学

神经解剖学杂志

CSTPCDCSCD北大核心
影响因子：0.538
ISSN：1000-7547
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