首页|Data driven analysis reveals prognostic genes and immunological targets in human sepsis-associated acute kidney injury

Data driven analysis reveals prognostic genes and immunological targets in human sepsis-associated acute kidney injury

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BACKGROUND:The molecular mechanism of sepsis-associated acute kidney injury(SA-AKI)is unclear.We analyzed co-differentially expressed genes(co-DEGs)to elucidate the underlying mechanism and intervention targets of SA-AKI.METHODS:The microarray datasets GSE65682,GSE30718,and GSE174220 were downloaded from the Gene Expression Omnibus(GEO)database.We identified the co-DEGs and constructed a gene co-expression network to screen the hub genes.We analyzed immune correlations and disease correlations and performed functional annotation of the hub genes.We also performed single-cell and microenvironment analyses and investigated the enrichment pathways and the main transcription factors.Finally,we conducted a correlation analysis to evaluate the role of the hub genes.RESULTS:Interleukin 32(IL32)was identified as the hub gene in SA-AKI,and the main enriched signaling pathways were associated with hemopoiesis,cellular response to cytokine stimulus,inflammatory response,and regulation of kidney development.Additionally,IL32 was significantly associated with mortality in SA-AKI patients.Monocytes,macrophages,T cells,and NK cells were closely related to IL32 and were involved in the immune microenvironment in SA-AKI patients.IL32 expression increased significantly in the kidney of septic mouse.Toll-like receptor 2(TLR2)was significantly and negatively correlated with IL32.CONCLUSION:IL32 is the key gene involved in SA-AKI and is significantly associated with prognosis.TLR2 and relevant immune cells are closely related to key genes.

SepsisAcute kidney injuryInterleukin 32Toll-like receptor 2Bioinformatics analysis

Qing Zhao、Jinfu Ma、Jianguo Xiao、Zhe Feng、Hui Liu

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Department of Diagnosis and Treatment of Cadres,the First Medical Center,Chinese PLA General Hospital,Beijing 100853,China

Intensive Care Unit,the 305th Hospital of Chinese PLA,Beijing 100032,China

Department of Critical Care Medicine,the First Medical Center,Chinese PLA General Hospital,Beijing 100853,China

Nephrology Department,the First Medical Center,Chinese PLA General Hospital,Beijing 100853,China

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Beijing Natural Science Foundation

7222162

2024

10.5847/wjem.j.1920-8642.2024.026

世界急诊医学杂志(英文)
浙江大学,浙江大学医学院附属第二医院

世界急诊医学杂志(英文)

ISSN：1920-8642
年,卷(期)：2024.15(2)
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