In order to explore anti-bacterial strains,the study on the inhibit function of the endophytic actinomycetes and their fermentation liquid on Staphylococcus aureus,Escherichia coil and rot-knot nematodes is conducted. The results show that S. aureus and E. coli could be inhibited effectively by the body and their fermentation broth of endophytic actinomycetes YI2 and A7 respectively. The inhibition diameters of Y12 is 15 mm and that of AT is 21 mm.S. aureus and E. coli can be inhibited by Y7 at the same time. But they couldn't kill or inhibit rotknot nematodes. It's indicated that there is not relation between the inhibition function of pathogenic bacteria and root-knot nematodes.