This study aims to establish a qualitative and quantitative analysis method for simultaneous determination of active ingredients in prothioconazole·epoxiconazole 30％SC using a high-performance liquid chromatography(HPLC).A chromatographic column with a filling material of 5TC-C18 and a UV detector with variable wavelength were used,and a methanol pH 3.0 phosphoric acid aqueous solution with a volume ratio of 80:20 was used as the mobile phase.The results showed that the linear correlation coefficients of prothioconazole and epoxiconazole were 0.9999 and 0.9996,the coefficient of variations were 0.1％and 0.2％,the average recoveries were 99.76％and 99.90％,and the standard deviations were 0.02 and 0.02.This method is simple,rapid,accurate,and has good reproducibility,and can meet the requirements for quantitative analysis of prothioconazole and epoxiconazole mixtures.
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