Determination of propoxur and beta-cypermethrin in water by HPLC-MS/MS
何伟 1何钰2
扫码查看
点击上方二维码区域，可以放大扫码查看
作者信息
1. 贵州省检测技术研究应用中心,贵阳 550002
2. 贵州省分析测试研究院,贵阳 550002
折叠
摘要
利用高效液相色谱-三重四极杆质谱仪建立水体中残杀威和高效氯氰菊酯含量检测方法.采用高效液相色谱-串联质谱法,以乙腈-5 mmol/L乙酸铵水溶液(90％+10％)为流动相,使用以Eclipse Plus C18填充的色谱柱和质谱检测器,对水体中的残杀威和高效氯氰菊酯进行测定,外标法定量.结果表明:残杀威和高效氯氰菊酯分别在0.94～47.10、0.63～31.29 μg/L质量浓度范围内线性关系良好,相关系数分别为0.9991和0.9994,变异系数分别为0.52％和 2.05％,高质量浓度水平的平均回收率分别为102.26％和102.44％,低质量浓度水平的平均回收率分别为90.74％和 98.24％.该方法具有操作简便、分离效果好、快速等优点,线性关系、准确度、精密度和灵敏度均满足要求,可用于水体中残留检测和水生生态环境毒理研究.
Abstract
This study aims to establish a high performance liquid chromatography triple quadrupole mass spectrometry for detecting the content of propoxur and beta-cypermethrin in water.Using HPLC-MS/MS method,with mobile phase of acetonitrile-5 mmol/L ammonium acetate aqueous solution(90％+10％),with column by Eclipse Plus C18 packing and MSD detector,propoxur and beta-cypermethrin were determined and quantified by external standard method.The results showed that the linear correlation coefficient between comcentrations of propoxur and beta-cypermethrin and their responding values were 0.9991 and 0.9994 in the mass concentration range of 0.94-47.10 and 0.63-31.29 μ g/L,the variation coefficient were 0.52％and 2.05％,the average recovery rates of high concentration levels were 102.26％and 102.44％,the average recovery rates of low concentration levels were 90.74％and 98.24％,respectively.The method is simple,effective separation and fast,and its linear relationship,accuracy,precision,and sensitivity all meet the requirements.It can be used for residue detection in water and toxicology research of aquatic ecological environment.
维普
万方数据