The separation and quantification of prochloraz and carbendazim in samples were carried out by HPLC with acetonitrile:ammonium acetate aqueous solution as mobile phase,Eclipse XDB-C8 4.6×250 mm,5 μm column and ultraviolet detector(235 nm).The results showed that the linear correlation coefficients of the method were 0.9993 and 0.9998,the standard deviations were 0.10%and 0.03%,the coefficients of variation were 0.36%and 0.40%,and the average recoveries were 99.6%and 99.5%respectively.The analysis method has the advantages of simple operation,rapidness and the like,and can simultaneously detect two effective components,thereby greatly reducing the analysis time and cost.