The contents of tembotrione,nicosulfuron and terbuthylazine in tembotrione·nicosulfuron·terbuthylazine 26％OD were separated and quantitatively analyzed by HPLC with acetonitrile and phosphoric acid aqueous solution(pH 3.0)as the mobile phase,a Ci8 stainless steel column and a diode array detector at a wavelength of 235 nm.The results showed that under the chromatographic conditions,there was a good linear correlation between the mass concentrations of nicosulfuron,tembotrione and terbuthylazine and their corresponding peak area response values.The regression equations were y=96 525.755x+5.2106,y=38 721.7727x+3.5122 and y=97 558.0497x+6.9862,respectively,and the correlation coefficients were all 0.9999.The standard deviations of the determination results of nicosulfuron,tembotrione and terbuthylazine in 5 batches of samples were 0.01,0.03 and 0.08,respectively;the coefficients of variation were 0.54％,0.75％and 0.39％,respectively;the average recoveries of the blank samples added with nicosulfuron,tembotrione and terbuthylazine standards were 99.80％,99.73％and 99.94％,respectively.This indicates that this method is applicable to the quality control requirements of the production process of tembotrione·nicosulfuron·tembotrione 26％OD products.
维普
万方数据