Cloning and Expression Analysis of QtSKOR2 Gene of Qiongzhuea tumidinoda Potassium Ion Channel
To investigate the function of the SKOR family in potassium ion transport in Qiongzhuea tumidinoda,a gene named QtSKOR2 is cloned from the young seedlings of Q.tumidinoda using cloning technology,and its physicochemical properties and expression pattern are analyzed.The result shows that the full length of the QtSKOR2 gene is 2 334 bp,with an open reading frame of 2 139 bp,which encodes 712 amino acids.Moreover,the QtSKOR2 protein has six transmembrane domains and exhibited the highest homology similarity with that of Phragmites australis at 90.46%.Cluster analysis suggests that the QtSKOR2 protein is most closely related to that of Oryza brachyantha.Additionally,the QtSKOR2 gene is expressed in all tissues of Q.tumidinoda,with the highest expression level in roots,followed by shoots,leaves,and culms.The expression level of the QtSKOR2 gene is highest in the roots of Q.tumidinoda and increases over time under 50 mmol/L KCl stress treatment.Additionally,the expression of the QtSKOR2 gene leaves is highest in Q.tumidinoda and increases over time under 250 mmol/L NaCl stress treatment.The results of this study reveal that the QtSKOR2 gene might play a significant role in Q.tumidinoda potassium ion transport under salt stress.
国家科技期刊平台
NSTL
万方数据
