Effects of Acupuncture Combined with Shenlingbaizhu Powder on Inflammation and Oxidative Stress Response of Rats with Ulcerative Colitis by Regulating Nrf2/HO-1 Pathway
Objective To observe the effects of acupuncture combined with Shenlingbaizhu Powder on inflamma-tion and oxidative stress response of rats with ulcerative colitis by the positive regulation of nuclear factor related factor 2(Nrf2)/heme oxygenase-1(HO-1)pathway.Methods A total of 60 SPF-grade healthy male SD rats were divided in-to control group(10 rats)and modeling group(50 rats).No special treatment was given to the control group,while the mod-eling group was treated with TNBS/ethanol enema.After successful modeling,the rats were divided into model group,acu-puncture group,Shenlingbaizhu powder group,combined group and western medicine group using a random number table,with 10 rats in each group.After 14 days of treatment,disease activity index(DAI)and Colon macroscopic damage index(CMDI)were evaluated.The contents of tumor necrosis factor-α(TNF-α),interleukin-1 β(IL-1 β),interleukin-6(IL-6),interleukin-18(IL-18),reactive oxygen species(ROS),malondialdehyde(MDA),superoxide dismutase(SOD),and glutathione peroxidase(GSH-Px),as well as the expression levels of Nrf2 and HO-1 in intestinal mucosa were detected.Results The DAI,CMDI,and the contents of TNF-α,IL-1β,IL-6,IL-18,ROS and MDA in intestinal mucosal tissues of model group were higher than those of control group,while the contents of GSH-Px and SOD,as well as the expression levels of Nrf2 and HO-1 in intestinal mucosal tissues of model group were lower than those of control group(P＜0.05).The DAI,CMDI,and the contents of TNF-α,IL-1β,IL-6,IL-18,ROS and MDA in the intestinal muco-sal tissue of rats in acupuncture group,Shenlingbaizhu powder group,combined group and western medicine group were lower than those in model group,and the contents of GSH-Px and SOD,as well asthe expression levels of Nrf2 and HO-1 in intestinal mucosa were higher than those in model group,and the differences were statistically significant(P＜0.05).The improvement in the combined treatment group were more significant than that in the acupuncture group,Shenlingbaizhu powder group and western medicine group,and the differences were statistically significant(P＜0.05).Conclusion Acu-puncture combined with Shenlingbaizhu powder can improve inflammation and oxidative stress in UC rats,and its mecha-nism may be related to positive regulation of Nrf2/HO-1 pathway.
Ulcerative colitisAcupunctureShenlingbaizhu PowderNuclear factor related factor 2Heme oxygen-ase-1
万方数据
维普
