Analysis of Key Immune Gene Mechanism and Screening of Traditional Chinese Medicine in Ulcerative Colitis
Objective Based on bioinformatics technology,the key immune target genes of ulcerative colitis(UC)were screened,and the related mechanism was analyzed.The effective traditional Chinese medicine(TCM)was screened,so as to provide theoretical support for basic research of UC and precise TCM treatment based on syndrome differentiation.Methods Key immune target genes against UC were screened by the GEO dataset,ImmPort database,SRTING database,cytoHubba plug-in,etc.The target genes were subjected to logistic univariate analysis,correlation analysis by applying the cor function,immune infiltration analysis by applying the CIBERSORT algorithm,and KEGG and GO enrichment analysis by applying the Enrichr database.miRNA was screened by searching miRWalk,RNA22,RNAInter,and TargetScan data-bases,and potential TCM was screened by the Coremine Medical database.Results Fifteen target genes with upregulated expression were screened,all of which were independent risk factors for UC.Immune infiltration analysis showed that CD4+memory-activated T cells,M0 macrophages,Ml macrophages,and neutrophils are differentially expressed between groups,and the target genes were closely associated with immune cells.KEGG analysis showed that the IL-17 signaling pathway and TNF signaling pathway were important pathways,and GO analysis indicated that biological processes mainly occurred in neutrophil chemotaxis and granulocyte chemotaxis.The cell components mainly consisted of a tertiary granule lumen and an extracellular matrix containing collagen,and the molecular functions were mainly manifested in the aspects of chemokine activity and chemokine receptor binding.The nine screened miRNAs had potential regulatory relationships with target genes,and the screened TCM such as Scutellaria baicalensis,Radix Ginseng,and Radix Curcumae had the potential for multi-target treatment of UC.Conclusions The immune mechanism of UC is complex and is the result of synergistic effects of multiple factors,targets,and pathways.By analyzing the key immune genes against UC,this study is helpful to explore the pathogenesis of UC,discover the potential targets and clinical value,and provide new perspectives for basic research and improvement of effective treatment protocols.
Ulcerative ColitisImmune-Related GenesBioinformaticsImmune InfiltrationSignaling PathwaymiRNATraditional Chinese Medicine
NETL
NSTL
万方数据
