Effects of Xinkeshu Tablet on Cardiac Function in Rats with Heart Failure with Pre-served Ejection Fraction and Its Mechanism
Objective To observe the effects of the Xinkeshu(XKS)Tablet on cardiac structure and function in rats with heart failure with preserved ejection fraction(HFpEF).Methods Thirty spontaneously hypertensive rats(SHRs)were divided into the SHR group,HFpEF group,and XKS group(334.8 mg/kg),and ten Wistar Kyoto(WKY)rats were used as the control group.The XKS group was given the corresponding drugs by gavage,and the other groups were given the same amount of normal saline for four weeks.Blood pressure and heart mass index(HMI)were measured.Echocardiography was used to detect the left atrial diameter(LAD),interventricular septal thickness(IVST),left ventricular end-diastolic diameter(LVEDd),left ventricular posterior wall thickness(LVWPd),E/A,E/E',isovolumetric relaxation time(IVRT),and left ventricular ejection fraction(LVEF).The serum levels of brain natriuretic peptide(BNP),angiotensin ar ejection fraction(LVEF thickness(IVST),left ventricular end-diastolic diameter(LVEDd),left ventricular posterior wa immu-nosorbent assay.HE and Masson staining were used to observe the pathological changes in myocardial tissue.Results Compared with the WKY group,the blood pressure and HMI of the HFpEF model group were increased,and the difference was statistically significant(P＜0.05).LAD,LVEDd,IVST,LVPWd,E/A,E/E',and IVRT were increased(P＜0.05),but LVEF did not change significantly,with the difference not statistically significant(P＞0.05).Pathological observation showed that the left atrium and left ventricle had enlarged cardiomyocytes and myocardial fibrosis.The serum levels of BNP,Ang Ⅱ,ALD,and sST2 were increased(P＜0.05),suggesting that the model was successfully established.Compared with the HFpEF group,systolic blood pressure and HMI were decreased in the XKS group(P＜0.05).LAD,LVEDd,IVST,LVPWd,E/A,E/E',and IVRT were decreased(P＜0.05).The pathological observation showed a reduction in car-diomyocytes and an improvement in myocardial fibrosis of the left atrium and ventricle.The serum levels of BNP,Ang Ⅱ,ALD,and sST2 were decreased(P＜0.05).Conclusion XKS Tablet can improve the structure and function of the left a-trium and ventricle in HFpEF rats,and its mechanism may be related to regulating the renin-angiotensin-aldosterone system and the expression of sST2,thereby improving myocardial hypertrophy and fibrosis.
Xinkeshu TabletHeart Failure with Preserved Ejection FractionHFpEF
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