Mechanism of Mashe Anjin Pills in the Treatment of Chronic Obstructive Pulmona-ry Disease Based on Network Pharmacology
Objective To explore the potential mechanism of Mashe Anjin Pills in the treatment of chronic ob-structive pulmonary disease(COPD)based on network pharmacology.Methods The active components and corresponding targets of Mashe Anjin Pills were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and A-nalysis Platform(TCMSP)and related articles,on the basis of which the drug-active component-target network of Mashe Anjin Pills was established.The targets related to COPD were retrieved from Drugbank and Genecards,and the common tar-gets shared by COPD and the active components of Mashe Anjin Pills were selected as the potential treatment targets.Metascape and DAVID were used for GO and KEGG pathway enrichment of the treatment targets,and the protein-protein interaction(PPI)network was established.Cytoscape was employed to analyze the topology of the PPI network.Results A total of 1 525 active components in Mashe Anjin Pills were retrieved,including 22 in Pinelliae Rhizoma,18 in Paeoniaera-dix Rubra,22 in Chuanxiong Rhizoma,95 in Glycyrrhizae Radix et Rhizoma,3 in Bombyx Batryticatus,26 in Armeniacae Semen Amarum,43 in Ephedrae Herba,1 in Ostreae Concha,29 in Aucklandiae Radix,23 in Amomi Fructus,12 in Belam-candae Rhizoma,and 5 in Fritillariae Thunbergii Bulbus,which corresponded to 298 targets.Among the top 10 important active components in the drug-active component-target network,6 were from Ephedrae Herba,3 from Paeoniaeradix Rubra,Bombyx Batryticatus,and Armeniacae Semen Amarum,2 from Belamcandae Rhizoma,Glycyrrhizae Radix et Rhizo-ma,Pinelliae Rhizoma,Chuanxiong Rhizoma,and Amomi Fructus,and 1 from Fritillariae Thunbergii Bulbus.KEGG enrich-ment analysis revealed the top 10 of the 20 modulated signal pathways.GO enrichment results showed that the top 10 bio-logical processes included enzyme binding and DNA-dependent positive transcriptional regulation;the top 10 cellular components included cytoplasm and cytoplasm;the top 10 molecular functions included protein denaturation regulation and protein metabolism regulation.The PPI network contained 21 nodes,among which JUN,MAPK1,MAPK3,TNF,RELA,MYC,STAT3,TP53,AKT1,MAPK8,EGFR,MAPK14,and PRKCA were the core targets.The animal experiments showed that the levels of interleukin(IL)-1β,1L-6,and tumor necrosis factor(TNF)-α in the serum followed the trend of con-trol group＞model group＞blank group(P＜0.05).The mRNA levels of MAPK1,MAPK3,and MYC also followed the trend of control group＞model group＞blank group(P＜0.05).Conclusion Majshe Anjin Pills can treat COPD through multiple components,targets,and pathways.The potential active components of Majshe Anjin Pills mainly come from Ephedrae Herbd,Belamcandae Rhizoma,Bupleuri Radix,Paeoniae Radix Alba,Atractylodis Macrocephalae Rhizoma,and Glycyrrhizae Radix et Rhizoma,and the core targets of Majshe Anjin Pills in treating COPD mainly include JUN,MAPK1,and MAPK3.
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