Intervention Effect of Weifuchun on Precancerous Lesions of Gastric Cancer Rats Based on PI3K/Akt Signaling Pathway
Objective To observe the effect of Weifuchun on precancerous lesions of gastric cancer rats(PLGC).Methods Thirty healthy,SPF,male Wistar rats were selected and divided into a Weifuchun group(n=10),a model group(n=10),and a control group(n=10)by random number table.The Weifuchun group received Weifuchun via ga-vage,while the model and control groups were administered saline or corresponding drug treatment,once daily for 16 weeks.Following successful modeling,all rats were euthanized,and gastric mucosa tissues were obtained for histopathologi-cal analysis.The expressions of Akt,p-Akt,NF-κB,and COX-2 in tissue samples were detected using RT-PCR and Western Blot.Results Weifuchun improved the general condition and macroscopic features of the PLGC rat model,and reversed the pathological state of the gastric mucosa,thus reducing the incidence of PLGC.Compared with the conditions in the control group,the protein expressions of Akt,p-Akt,NF-κB,and COX-2 in the model group were increased(P＜0.05).The Weifuchun group had decreased levels of these proteins compared with the model group(P＜0.05).Similarly,the mRNA expressions of Akt,p-Akt,NF-κB,and COX-2 were elevated in the model group compared with the control group(P＜0.05),while reduced in the Weifuchun group compared with the model group(P＜0.05).Conclusion Weifu-chun may regulate the abnormal activation of the PI3K/Akt signaling pathway by inhibiting the proliferation of Akt,p-Akt,NF-κB,and COX-2,thus preventing the occurrence and progression of PLGC.
Precancerous lesions of gastric cancerWeifuchunAktp-AktNF-κBCOX-2
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