Pathogenesis of Chronic Body Surface Ulcers Based on Oxidative Stress Responses and Intervention Effect of Traditional Chinese Medicine
Objective To explore the pathogenesis of chronic body surface ulcers and the intervention effect of Xiangpi Shengji Ointment combined with Tuoli Xiaodu Powder.Methods A total of 30 SPF-level male SD rats were ran-domly divided into the blank control group,model group,Xiangpi Shengji Ointment group,Tuoli Xiaodu Powder group,and Xiangpi Shengji Ointment combined with Tuoli Xiaodu Powder group,with six rats in each group.The model was made with Staphylococcus aureus.After one week of continuous administration,the expression levels of vascular endothelial growth factor(VEGF),vascular endothelial growth factor receptor 2(VEGFR-2),nuclear factor erythroid 2-related factor 2(Nrf2),NAD(P)H quinone oxidoreductase1(NQO1),and heme oxygenase 1(HO-1)in wound tissue were detected.Re-sults Compared with the blank control group,the expression of VEGF and VEGFR-2 in the wound tissue of the model group decreased significantly,and the difference was statistically significant(P＜0.01).Compared with the model group,the expression of VEGF and VEGFR-2 in the Xiangpi Shengji Ointment group,Tuoli Xiaodu Powder group,and Xiangpi Shengji Ointment combined with Tuoli Xiaodu Powder group were increased significantly(P＜0.01).Compared with the blank control group,the expression levels of Nrf2,NQO1,and HO-1 in the model group increased(P＜0.01).Compared with the model group,the expression level of Nrf2 in the Xiangpi Shengji Ointment group,Tuoli Xiaodu Powder group,and Xiangpi Shengji Ointment combined with Tuoli Xiaodu Powder group increased significantly,while the expression of NQO1 and HO-1 decreased(P＜0.01).Conclusion The formation of chronic body surface ulcers in rats may be related to the inhibition of the VEGF pathway and Nrf2 pathway by oxidative stress responses,and Xiangpi Shengji Ointment combined with Tuoli Xiaodu Powder may promote angiogenesis and anti-oxidation stress in the skin ulcer of rats by regulating the a-bove-mentioned pathways,so as to improve the healing ability of the wounds.
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