Changes of Serum Inflammatory Factors in Patients with Irritable Bowel Syndrome with Diarrhea and the Relationship with Traditional Chinese Medicine Syndrome Types
Objective To analyze the changes of serum inflammatory factors Interleukin-4(IL-4),IL-6,IL-8,IL-10,tumor necrosis factor-alpha(TNF-α)and interferon-gamma(IFN-γ)levels in patients with irritable bowel syndrome with diarrhea(IBS-D),and to explore the relationship between traditional Chinese medicine(TCM)syndrome types of IBS-D and serum inflammatory factors.Methods A total of 42 IBS-D patients and 30 healthy volunteers were included in this study.According to the diagnostic criteria,in IBS-D group,there were 9 cases of stagnation of liver qi and spleen deficiency,8 cases of weakness of the spleen and the stomach,5 cases of spleen-kidney yang deficiency syndrome,and 20 cases of large intestine damp-heat syndrome.They can be further divided into 22 cases of non-damp-heat syn-drome,and 20 cases of damp-heat syndrome.Clinical data and serum samples from the patients and healthy volunteers were collected,and serum inflammatory factor levels were detected by enzyme-linked immunosorbent assay(ELISA).Re-sults The serum level of IL-4 in IBS-D group was lower than that in healthy control group(P＜0.05)and the level of TNF-α in IBS-D group was higher than that in healthy control group(P＜0.05).There was no significant difference in serum IL-6,IL-8,IL-10 and IFN-γ levels between the two groups(P＞0.05).There were significant differences in serum IL-8 levels among different syndrome types(P＜0.01).The level of serum IL-8 in patients with stagnation of liver qi and spleen deficiency was significantly higher than that in patients with weakness of the spleen and the stomach(P＜0.01).The level of serum IL-8 in patients with large intestine damp-heat syndrome was significantly higher than that in patients with weakness of the spleen and the stomach(P＜0.01).There were significant differences in serum IL-8 levels among damp-heat syndrome,non-damp-heat syndrome and healthy control groups(P＜0.05).The level of serum IL-8 in patients with damp-heat syndrome was higher than that in patients without damp-heat syndrome(P＜0.05).The serum levels of TNF-α and IFN-γ in patients with damp-heat syndrome were higher than those in patients without damp-heat syndrome and the level of serum IL-10 in patients with damp-heat syndrome was lower than that in patients without damp-heat syndrome,but there were no significant difference.The level of serum TNF-α in patients with damp-heat syndrome was significantly higher than that in the healthy control group(P＜0.05).Conclusion The low-grade inflammation of IBS-D patients may be related to the decreased level of anti-inflammatory factors and increased level of pro-inflammatory factors in the serum.There are differences in serum levels of inflammatory factors among different TCM Syndrome types of IBS-D,and damp-heat syndrome may be related to the low-grade inflammatory state and immune activation of IBS-D.
Irritable Bowel Syndrome with DiarrheaInflammatory FactorsSyndrome Types
维普
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