Serum Metabonomics Study on Mechanism of Action of Yangjin Jiedu Decoction on Diabetic Peripheral Neuropathy Rats
Objective To study the mechanism of Yangjin Jiedu Decoction(YJJD)in treating diabetic peripheral peripheral neuropathy(DPN)rats based on serum metabolomics.Methods 24 male SPF-grade SD rats were randomly divided into a control group,a model group,and a YJJD group,with 8 rats in each group.Rats were fed with the high-fat and high-sugar diet for 8 weeks except for the control group,with streptozotocin(stz)injected intraperitoneally to make the DPN model.After 8 weeks of a high-fat and high-sugar diet,rats were given YJJD(0.248 g/kg),twice daily for 8 weeks.Blood samples were collected to measure the fasting blood glucose(Glu)of rats,and serum was isolated using non-targeted metabonomics.Principal component analysis(PC A)and orthogonal partial least squares discriminant analysis(OPLS-DA)combined with t-test,variable importance projection,and differential fold values to screen the differential metabolites and possible pathways.Results YJJD significantly decreased the blood glucose level of DPN rats compared with the control group.Through metabolomics,it was found that YJJD could obviously improve 148 different metabolites,in-cluding Pro-leu,Pro-Ile,and Leu-Tyr,and also influence steroid hormone biosynthesis,arginine and proline metabo-lism,glycine,serine and threonine metabolism and other metabolic pathways.Conclusion YJJD has a certain therapeutic effect on DPN rats,and its mechanism may be related to the regulation of amino acid and corticosteroid metabolism.