Effects of Salidroside on Lung Adenocarcinoma A549 Cells via Wnt/β-catenin Sig-naling Pathway
Objective This study aims to investigate the effects of salidroside on the proliferation,migration,cell cycle,and apoptosis of lung adenocarcinoma A549 cells,as well as the mechanism of action of salidroside.Methods The human lung adenocarcinoma A549 cells were treated with salidroside,and the effect of salidroside on cell proliferation and migration was detected by cell scratch assay and Transwell assay.Cell apoptosis was detected by Hoechst 33342 fluorescent staining assay and Annexin V-FITC/PI double staining assay.The cell cycle was detected by propidium iodide staining.The protein expressions of Bax,Bcl2,P21,CyclinD1,Wnt3a,and β-catenin were detected by Western blot.The gene ex-pression of Bax,Bcl2,P21,CyclinD1,Wnt3a,and β-catenin was detected by real-time quantitative polymerase chain re-action(PCR).Results Salidroside could inhibit the proliferation and migration of A549 cells,make cell cycle arrest in the G1/G2 phase,induce apoptosis,promote the expression of pro-apoptotic proteins and genes,and inhibit the expression of anti-apoptotic proteins and genes and the expression of Wnt3a and β-catenin proteins and genes related to transcrip-tion.Conclusion Salidroside could inhibit proliferation,migration,and cell cycle and induce apoptosis in lung adenocarci-noma A549 cells,which might be related to the down-regulation of the Wnt/β-catenin signaling pathway.
万方数据
维普
