首页|基于HIF-1α探讨七龙天对高原低氧模型大鼠肺损伤的保护作用及机制

基于HIF-1α探讨七龙天对高原低氧模型大鼠肺损伤的保护作用及机制

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目的 探讨七龙天基于低氧诱导因子-1α(Hypoxia inducible factor-1α,HIF-1α)对高原低氧模型大鼠肺损伤的保护作用及机制.方法 将45只SPF级SD雄性大鼠按完全随机分组法分为空白组、模型组、七龙天组,每组各15只.除空白组外,造模前3 d给药,连续3 d,采用低压氧仓构建高原低氧模型,连续72 h.测定左肺肺组织含水量,以RT-qPCR检测肺组织中HIF-1α、血管内皮生长因子(Vascular endothelial growth factor,VEGF)、胞红蛋白(Cytoglobin,CGB),促红细胞生成素(Erythropoietin,EPO),基质金属蛋白酶 9(Matrix metallopro-teinase-9,MMP9)、组织金属蛋白酶抑制因子1(TIMP metallopeptidase inhibitor 1,TIMP1)的mRNA表达,免疫组化检测HIF-1α、VEGF,CGB,EPO的蛋白表达,Western blot检测MMP9、TIMP1的蛋白表达.结果 模型组肺组织含水量较空白组明显升高,与模型组比较,七龙天组可明显降低肺组织含水量;模型组HIF-1α、VEGF、CGB、EPO,MMP9、TIMP1的mRNA与蛋白表达均明显升高,七龙天组可明显降低HIF-1α、VEGF、CGB、EPO,MMP9、TIMP1的mRNA与蛋白表达.结论 七龙天可降低肺组织含水量,其干预机制与抗氧化应激,HIF-1α调控下游VEGF、MMP9有关,待进一步验证.
Exploring the Protective effects and Mechanism of Qilongtian on Lung injury in High-Altitude Hypoxia Model Rats Based on HIF-1α
Objective To explore the protective effects and mechanism of Qilongtian on lung injury in high-alti-tude hypoxia model rats,focusing on the role of hypoxia-inducible factor-1α(HIF-1α).Methods Forty-five SPF-grade SD male rats were randomly divided into three groups:blank group,model group and Qilongtian group,with 15 rats in each group.Except for the blank group,all rats were administered Qilongtian for 3 consecutive days before modeling.A high-altitude hypoxia model was established using a hypobaric oxygen chamber for 72 hours.The water content of left lung tis-sue was measured,and RT-qPCR was used to detect mRNA expression levels of HIF-1α,vascular endothelial growth factor(VEGF),cytoglobin(CGB),erythropoietin(EPO),matrix metalloproteinase-9(MMP9),and tissue inhibitor of met-alloproteinases 1(TIMP1)in lung tissue.Immunohistochemistry was used to detect protein expression of HIF-1α,VEGF,CGB,and EPO.Western blot was performed to analyze protein expression levels of MMP9 and TIMP1.Results The model group showed a significant increase in lung tissue water content compared to the blank group.In contrast,the Qilongtian group showed a significant reduction in lung tissue water content compared to the model group.The mRNA and protein ex-pression levels of HIF-1α,VEGF,CGB,EPO,MMP9,and TIMP1 were significantly elevated in the model group,while Qilongtian treatment significantly reduced these levels.Conclusion Qilongtian can reduce the water content in lung tis-sue,and its protective mechanism may involve the mitigation of oxidative stress and the regulation of downstream factors such as VEGF and MMP9 via HIF-1α.Further studies are needed to confirm these findings.

QilongtianHigh Altitude HypoxiaHigh Altitude Pulmonary EdemaHypoxia-Inducible Factor-1α

付兴、付义

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海南医科大学,海南海口 571199

昆明市中医医院,云南昆明 650599

七龙天 高原低氧 高原肺水肿 低氧诱导因子-1α

国家自然科学基金项目国家自然科学基金项目国家自然科学基金项目海南省自然科学基金资助

821046388226092482274374824MS068

2024

世界中西医结合杂志
中华中医药学会

世界中西医结合杂志

CSTPCD
影响因子:1.053
ISSN:1673-6613
年,卷(期):2024.19(8)
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