Effects and Mechanism of Jinzhen Oral Liquid on Airway Neurogenic Inflammation in Cough Hypersensitivity Guinea Pigs
Objective To explore the effects and mechanism of Jinzhen Oral Liquid(JZOL)on airway neurogenic inflammation and cough in a guinea pig model with cough hypersensitivity.Methods Forty-eight guinea pigs were ran-domly divided into six groups(n=8 per group):the model group,blank control group,high-dose JZOL group,medium-dose JZOL group,low-dose JZOL group,and amoxicillin group.After establishing the cough hypersensitivity model,cough sensitivity was observed post-administration in each group.Serum levels of immunoglobulin E(IgE)and interleukin-5(IL-5)were measured using enzyme-linked immunosorbent assay(ELISA).Quantitative real-time PCR(qPCR)was used to assess the mRNA expression levels of transient receptor potential ankyrin 1(TRPA1),substance P(SP),neuroki-nin A(NKA),and eotaxin(EOT)in tracheal tissues.Hematoxylin-eosin(HE)staining was conducted to observe the path-ological changes in lung tissues.Results Compared with the model group,JZOL significantly reduced cough sensitivity.Although there were no statistically significant differences in serum IgE and IL-5 levels,JZOL exhibited a notable down-ward trend in these markers,with stronger efficacy than amoxicillin.JZOL inhibited the expression of EOT,SP,NKA,and TRPA1 mRNA in tracheal tissue.Additionally,JZOL improved lung tissue pathology,with the severity of lesions decreasing in the following order:medium-dose JZOL group,amoxicillin group,low-dose JZOL group,and high-dose JZOL group.Conclusion JZOL may alleviate airway neurogenic inflammation by downregulating the expression of SP,NKA,and EOT through the TRPA1 pathway,reducing serum inflammatory factors and immunoglobulin levels.