Effects of Marsdeniae Tenacissimae Caulis on Src/FAK Signaling Pathway and Th1/Th2-Related Cytokine Levels in Mice with Gastric Cancer
Objective To investigate the effects of Marsdeniae Tenacissimae Caulis on steroid receptor coactivator(Src)/focal adhesion kinase(FAK)signaling pathway and Th1/Th2-related cytokine levels in a mouse model of gastric cancer.Methods A total of 60 adult SPF-grade C57BL/6 mice,equally divided between males and females,were ran-domly assigned to six groups using the random number table method:blank control group,model group(injected with sa-line),cyclophosphamide group(injected with cyclophosphamide),and three Marsdeniae Tenacissimae Caulis groups with low,medium,and high doses(injected with 0.1,0.2 and 0.4 mL,of Marsdeniae Tenacissimae Caulis extract,respective-ly),with 10 mice in each group.Interventions were administered once every 2 days.On the 21st day,all mice were sacri-ficed,and blood samples were collected from the neck.The body weight,thymus weight,spleen weight,and tumor weight of the mice were measured.HE staining was used to observe the pathological changes in the tumor tissue.Flow cytometry was used to detect the levels of CD4+,CD8+,and the CD4+/CD8+ratio.ELISA was used to measure the levels of interleukin-2(IL-2),interleukin-4(IL-4),interleukin-10(IL-10),and interferon-γ(INF-γ).Western blot analysis was used to detect the expression levels of Src,phosphorylated Src(p-Src),FAK,and phosphorylated FAK(p-FAK).Re-sults Compared with the model group,the spleen and thymus indices in the Marsdeniae Tenacissimae Caulis intervention groups increased,and these indices,along with the tumor inhibition rate,increased with the dosage of Marsdeniae Tenacis-simae Caulis,with statistically significant differences(P＜0.05).The spleen and thymus indices in the cyclophosphamide group were significantly lower than those in the Marsdeniae Tenacissimae Caulis groups,with statistically significant differ-ences(P＜0.05).The tumor inhibition rates in the cyclophosphamide and high-dose Marsdeniae Tenacissimae Caulis groups were significantly higher than those in the low-and medium-dose Marsdeniae Tenacissimae Caulis groups,with statistically significant differences(P＜0.05).HE staining revealed that the tumor cells in the model group were densely and orderly arranged,while the cyclophosphamide and Marsdeniae Tenacissimae Caulis groups showed reduced tumor cell density,uneven distribution,and varying degrees of necrosis.Compared with the model group,the levels of IL-2 and INF-γ increased and the levels of IL-4 and IL-10 decreased in the Marsdeniae Tenacissimae Caulis intervention groups,with statistically significant differences(P＜0.05).Compared with the cyclophosphamide group,the levels of IL-2 and INF-γincreased,and the levels of IL-4 and IL-10 decreased in the Marsdeniae Tenacissimae Caulis low-,medium-,and high-dose groups,with statistically significant differences(P＜0.05);the higher the intervention dose of Marsdeniae Tenacissimae Caulis,the more pronounced the increase in IL-2 and INF-γ levels,and the more pronounced the decrease in IL-4 and IL-10 levels(P＜0.05).Compared with the model group,the levels of CD4+and the CD4+/CD8+ratio in-creased,and CD8+levels decreased in the cyclophosphamide and Marsdeniae Tenacissimae Caulis groups,with statistically significant differences(P＜0.05).Compared with the cyclophosphamide group,the levels of CD4+and CD4+/CD8+ratio increased,and CD8+levels decreased in the Marsdeniae Tenacissimae Caulis low-,medium-,and high-dose groups,with statistically significant differences(P＜0.05);the higher the intervention dose of Marsdeniae Tenacissimae Caulis,the more pronounced the increase in CD4+and the CD4+/CD8+ratio,and the more pronounced decrease in CD8+levels(P＜0.05).Compared with the model group,the p-Src/Src and p-FAK/FAK protein levels decreased in the cyclophos-phamide and Marsdeniae Tenacissimae Caulis groups,with statistically significant differences(P＜0.05).Compared with the cyclophosphamide group,the p-Src/Src and p-FAK/FAK protein levels increased in the Marsdeniae Tenacissimae Caulis low-,medium-,and high-dose groups,with statistically significant differences(P＜0.05).Conclusion Mars-deniae Tenacissimae Caulis can modulate the Src/FAK signaling pathway and Th1/Th2-related cytokine levels in gastric cancer mice,thereby exerting an anti-cancer effect,with more significant effects observed at higher doses.
万方数据
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