Inhibition Effect of Guishen Pill on Intrauterine Adhesions by Regulating Epithelial-mesenchymal Transition Mediated by TGF-β/Snail Signaling Pathway
Objective To study the regulatory effects of Guishen Pill on fibrosis of primary endometrial epithelial cells(PEEC)and epithelial-mesenchymal transition(EMT)mediated by TGF-β/Snail signaling pathway and to elucidate the potential therapeutic effect of Guishen Pill on intrauterine adhesions and its molecular mechanism.Methods TGF-β1 was used to induce EMT of PEEC in vitro for 48 hours.Then the PEEC were treated with Guishen Pill containing serum for 24 hours.The mRNA and protein expression of TGF-β1 downstream gene Snail,EMT markers vimentin,E-cadherin,and N-cadherin,and cell fibrosis marker α-SMA were detected by quantitative polymerase chain reaction(qPCR)and Western blot(WB),respectively to characterize the therapeutic effect of Guishen Pill.Results The serum containing Guishen Pill was verified to have no cytotoxicity.TGF-β1 successfully induced the occurrence of EMT in PEEC,which was manifested as expression increases of Snail,vimentin,N-cadherin,and α-SMA and expression decrease of E-cad-herin.Guishen Pill containing serum inhibited the EMT in PEEC and reduced the fibrosis level of PEEC,which was reflec-ted by expression decreases of Snail,vimentin,N-cadherin,and α-SMA and expression increase of E-cadherin.Con-clusion Guishen Pill showed therapeutic effect on intrauterine adhesions by reducing fibrosis level of PEEC via inhibiting EMT mediated by TGF-β/Snail signaling pathway.
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维普
