Objective:To explore the potential active components and mechanism of Loki zupa(LKZP)for asthma treatment through pharmacodynamic evaluation,network pharmacology and molecular docking.Methods:The anti-asthmatic activity of LKZP was studied in ovalbumin(OVA)-induced asthma model mice.The chemical components of LKZP collected from literature and their targets were screened by Lipinsk's Rule of Five.After their intersection with the asthma targets,the anti-asthma targets of LKZP were determined.The core targets of LKZP for asthma treatment were determined by constructing a protein-protein interaction(PPI)network of therapeutic targets using the String.Then GO and KEGG enrichment analyses were proformed on the core targets by the DAVID.Finally,a component-target-pathway network was built by Cytoscape,and the binding energy of active components and tar-gets was calculated by molecular docking.Results:A total of 136 potential active compounds and 841 targets of LKZP were screened,with 66 core targets.According to the network analysis,the key targets of LKZP for asthma treatment were MAPK1,EG-FR,MAPK3,PIK3CA,AKT1,PIK3CB,PIK3CD,PIK3R1 and PRKCA.The signaling pathways were mainly related with the regula-tion of airway inflammation(such as PI3K-Akt signaling pathway,ErbB signaling pathway,and estrogen signaling)and the regula-tion of airway remodeling(such as HIF-1 signaling pathway,VEGF signaling pathway,and calcium signaling pathway).Finally,the molecular docking indicated a strong binding energy between key active components and targets.Conclusion:Sesquiterpenes,fla-vonoids and phenylpropanoids,the key active components in LKZP,regulate the airway inflammation and airway remodeling by mul-tiple targets and pathways,thereby treating asthma.
国家科技期刊平台
NSTL
维普
万方数据
