首页|糖耐康改善KKAy小鼠Toll样受体4通路及葡萄糖转运子4的作用机制研究

糖耐康改善KKAy小鼠Toll样受体4通路及葡萄糖转运子4的作用机制研究

Mechanism of Tangnai Kang in Improving TLR4 Signaling Pathway and GluT-4 in KKAy Diabetic Mice

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  • 国家科技期刊平台
  • NETL
  • NSTL
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目的:观察中药糖耐康对KKAy小鼠血糖、体质量及Toll样受体4(TLR4)通路中白细胞介素6(IL-6)、核因子κB激酶β抑制物(IKKβ)及葡萄糖转运蛋白4(GluT4)影响,分析其改善2型糖尿病的可能作用机制.方法:选取无特定病原体(SPF)级自发性2型糖尿病动物KKAy小鼠及C57BL/6J小鼠,给予高脂饲料喂养,连续8周给药,评价血糖及体质量.并采用半定量酶联免疫吸附测定(ELISA)检测血浆中IL-6及GluT4的水平.蛋白质印迹法检测肝组织TLR4蛋白表达、肌肉组织IKKβ蛋白表达水平.结果:中药糖耐康高剂量组,能够显著降低血糖水平(P＜0.05),具有一定抑制体质量增长的趋势.同时能够下调IL-6水平,上调GluT4的水平(P＜0.01,P＜0.05),抑制TLR4及IKKβ蛋白表达(P＜0.01,P＜0.05).结论:糖耐康可能通过下调脂多糖(LPS)介导的TLR4通路中关键炎症介质的水平,减轻低度炎症反应,在一定程度上抑制炎症级联效应,同时提高胰岛素信号转导受体后途径中GluT4的转位及活性水平,共同达到改善胰岛素抵抗状态的作用.
Objective:To observe the effects of traditional Chinese medicine(TCM)compound Tangnai Kang on blood sugar,body weight,interleukin-6(IL-6)and inhibitor of nuclear factor kappaB kinase beta(IKKβ)in Toll-like receptor 4(TLR4)signaling pathway and glucose transporter 4(GluT-4)in KKAy mice,and to discuss its possible mechanism in improving type 2 diabetes mel-litus(T2DM).Methods:SPF KKAy and C57BL/6J mice(spontaneous T2DM animals)were selected and fed with high fat food.After 8 weeks,blood sugar and body weight were evaluated and the levels of IL-6 and GluT-4 in plasma were measured by semi-quantitative ELISA.The protein expressions of TLR4 in liver tissue and IKKβin muscle tissue were detected by Western blot.Re-sults:High dose of Tangnai Kang reduced blood glucose(P＜0.05)and controlled the growth trend of body weight.It also de-creased the level of IL-6 and increased the level of GluT-4(P＜0.01,P＜0.05),and inhibited the protein expressions of TLR4 in liver tissue and IKKβ in muscle tissue(P＜0.01,P＜0.05).Conclusion:Tangnai Kang may reduce the low-grade inflammation response by down-regulating the key inflammatory factors in the LPS-mediated TLR4 signaling pathway,and thus inhibit the inflam-matory cascade effect to some extent.It also enhances the transposition and activity of GluT-4 in the insulin signal transduction pathway,to jointly improve the insulin resistance.

Tangnai KangSpontaneous diabetic KKAy miceToll-like receptor 4(TLR4)signaling pathwayInterleukin-6(IL-6)Inhibitor of nuclear factor kappaB kinase beta(IKKβ)Glucose transporter 4(GluT-4)Mechanism

王芬、何华亮、刘铜华、吴丽丽

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北京中医药大学第三附属医院,北京,100029

中国人民解放军武装警察部队北京总队医院,北京,100027

北京中医药大学教育部中医养生学重点实验室,北京,100029

糖耐康 自发性糖尿病小鼠 TLR4通路 白细胞介素6 核因子κB激酶β抑制物 葡萄糖转运蛋白4 作用机制

国家自然科学基金青年科学基金教育部优秀青年科学基金

820043382018-JYBZZ-JS169

2023

10.3969/j.issn.1673-7202.2023.19.003

世界中医药
世界中医药学会联合会

世界中医药

CSTPCDCHSSCD北大核心
影响因子：1.266
ISSN：1673-7202
年,卷(期)：2023.18(19)
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