滋阴补脾方对人结肠癌裸鼠移植瘤的抑制作用机制研究
Inhibitory Effect of Ziyin Bupi Decoction on Human Colon Cancer Xenografts in Nude Mice and Its Mechanism
武如通 1杨思华 1史清华 1张芸 1黎智燊 1郑周杭2
作者信息
- 1. 广东省第二中医院肿瘤科,广州,510095
- 2. 广州中医药大学,广州,510006
- 折叠
摘要
目的:探讨滋阴补脾方对人结肠癌裸鼠移植瘤的抑制作用机制.方法:4周龄BALB/c雌性裸鼠20只,制备人结肠癌裸鼠模型,按照随机数字表法分随机分为4组,每组5只.对照组注射等量生理盐水,化疗药物组腹腔注射氟尿嘧啶,中成药组灌胃中药汤剂10 mL,联合用药组:每只裸鼠腹腔注射氟尿嘧啶联合灌胃中药汤剂.1次/d,均连续应用4 d,至第14天处死动物收集标本.测量各组肿瘤重量、肿瘤体积和体积抑瘤率;采用蛋白质印迹法测定CD113、CD116和P53蛋白表达.结果:与对照组比较,化疗药物组、中成药组和联合用药组肿瘤重量、肿瘤体积、CD113和CD116表达明显降低,肿瘤体积抑瘤率和P53表达明显升高(P＜0.05);化疗药物组和联合用药组肿瘤重量、肿瘤体积、CD113和CD116表达低于中成药组,肿瘤体积抑瘤率和P53表达高于中成药组(P＜0.05);联合用药组肿瘤重量、肿瘤体积、CD113和CD116表达低于化疗药物组,肿瘤体积抑瘤率和P53表达高于化疗药物组(P＜0.05).结论:滋阴补脾法可抑制人结肠癌HT29裸鼠移植瘤生长,认为其机制可能与下调CD113和CD116表达及上调P53表达有关.
Abstract
Objective:To investigate the inhibitory effect and mechanism of Ziyin Bupi Decoction on human colon cancer xeno-grafts in nude mice.Methods:Twenty 1-month-old BALB/c female nude mice were used to establish a nude mouse model of human colon cancer,and they were randomly divided into four groups,with five mice in each group.The control group was injected with the same amount of normal saline,while the chemotherapy drug group was intraperitoneally injected with fluorouracil.The Chinese pa-tent medicine group was gavage with 10 mL of traditional Chinese medicine decoction,and the combined drug group was intraperito-neally injected with fluorouracil combined with traditional Chinese medicine decoction by gavage.Each group was treated once a day for consecutive four days,and the animals were killed on the 14th day to collect samples.The tumor weight,tumor volume,and tumor volume inhibition rate of each group were measured;the protein expressions of CD113,CD116,and P53 were determined by Western Blotting.Results:Compared with the control group,the tumor weight,tumor volume,and the expression of CD113 and CD116 in the chemotherapy drug group,Chinese patent medicine group,and combined drug group decreased significantly,and the tumor volume inhibition rate and the expression of P53 increased significantly(P＜0.05);the tumor weight,tumor volume,and the expression of CD113 and CD116 in the chemotherapy drug group and combined drug group were lower than those in the Chinese patent medicine group,and the tumor volume inhibition rate and P53 expression were higher than those in the Chinese patent medi-cine group(P＜0.05);the tumor weight,tumor volume,and expression of CD113 and CD116 in the combined drug group were lower than those in the chemotherapy drug group,and the tumor volume inhibition rate and P53 expression were higher than those in the chemotherapy drug group(P＜0.05).Conclusion:The Ziyin Bupi Decoction can inhibit the growth of human colon cancer HT29 xenografts in nude mice,and the mechanism may be related to the down-regulation of CD113 and CD116 expression and the up-regulation of P53 expression.
关键词
滋阴补脾方/氟尿嘧啶/人结肠癌/裸鼠/移植瘤/CD113蛋白/CD116蛋白/肿瘤蛋白P53Key words
Ziyin Bupi Decoction/Fluorouracil/Human colon cancer/Nude mice/Xenograft/CD113 protein/CD116 protein/Tumor protein P53引用本文复制引用
基金项目
广东省中医药局科研项目(20191033)
出版年
2023
国家科技期刊平台
NSTL
维普
万方数据