首页|推拿促进房水内源性前列腺素的生成调节大鼠睫状体淋巴管增生

推拿促进房水内源性前列腺素的生成调节大鼠睫状体淋巴管增生

Massage Regulates Lymphatic Hyperplasia in Ciliary Body of Rats by Promoting Generation of Aqueous Humor Prostaglandin

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 维普
  • 万方数据
目的:探讨推拿促进大鼠眼内前列腺素的生成,诱导大鼠眼部睫状体内淋巴管的增生,进而降低青光眼眼压的作用机制.方法:将48只SD大鼠造模后随机分为青光眼模型组、推拿组、拉坦前列腺素组和推拿结合拉坦前列腺素组,每组12例.治疗过程中分别抽取各组不同时间段房水进行酶联免疫吸附试验(ELISA),采用蛋白质印迹法、定量聚合酶链反应、免疫荧光法、免疫组织化学法检测同源盒基因转录因子1(Prox-1)、5'-核苷酸酶(5'-Nase)表达水平,淋巴管酶组织化学染色法检测各组睫状体内淋巴管的增生情况.结果:推拿结合拉坦前列腺素组眼压均低于其他组(P＜0.05);推拿结合拉坦前列腺素组中Prox-1、5'-Nase表达水平均高于其他组(P＜0.05);推拿结合拉坦前列腺素组睫状体组织淋巴增殖均高于其他组.结论:推拿可以有效促进睫状体淋巴管的增生,而增生的淋巴管能吸收多余的房水导致眼压下降.
Objective:To explore the mechanism of massage in reducing intraocular pressure in glaucoma by promoting the genera-tion of aqueous humor prostaglandin and inducing the intraocular lymphatic hyperplasia in rats.Methods:Forty-eight SD rats were randomized into glaucoma model,massage,latanoprost,and massage+latanoprost groups(n=12).The aqueous humor samples were collected from each group at different time points during the treatment period for enzyme-linked immunosorbent assay.Western blotting,qPCR,immunofluorescence assay,and immunohistochemical staining were employed to determine the expression levels of prospero homeobox 1(Prox-1)and 5'-Nase.Histochemical staining was employ to detect the lymphatic hyperplasia in the ciliary body in each group.Results:The massage+latanoprost group had lower intraocular pressure(P＜0.05),higher expression levels of Prox-1 and 5'-Nase(P＜0.05),and higher rate of lymphatic hyperplasia in the ciliary body than other groups.Conclusion:Mas-sage can promote the lymphatic hyperplasia in the ciliary body,which can facilitate the absorption of excess aqueous humor to re-duce intraocular pressure.

MassageAqueous humorProstaglandinLatanoprostIntraocular pressureCiliary bodyLymphatic vesselsGlau-coma

池华博文、李青松、贾琳琳、符之瑄、许愿、杨倩、杨俊倚

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上海中医药大学附属普陀医院,上海,200062

安徽医科大学附属普陀临床医学院,上海,200062

推拿 房水 前列腺素 拉坦前列腺素 眼压 睫状体 淋巴管 青光眼

国家自然科学基金上海市医学重点专科-眼科建设项目上海市普陀区卫生自主创新科研基金上海市普陀区自主创新重大项目卫生健康委临床研究课题(2020)

82074495A-26317-PT-0715-PT-01202040148

2023

10.3969/j.issn.1673-7202.2023.22.005

世界中医药
世界中医药学会联合会

世界中医药

CSTPCDCHSSCD北大核心
影响因子：1.266
ISSN：1673-7202
年,卷(期)：2023.18(22)
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