首页|金藤清痹颗粒对胶原诱导性类风湿关节炎大鼠滑膜血管新生的影响

金藤清痹颗粒对胶原诱导性类风湿关节炎大鼠滑膜血管新生的影响

Effect of Jinteng Qingbi Granule on Synovial Angiogenesis in Collagen-induced Arthritis Rats

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 维普
  • 万方数据
目的:观察金藤清痹颗粒对胶原诱导性类风湿关节炎(CIA)大鼠滑膜血管新生的治疗作用并探讨其作用机制.方法:取60只SD大鼠随机分为空白组,模型组,甲氨蝶呤组,金藤清痹颗粒低、中、高剂量组等6组.以免疫诱导方法建立CIA大鼠模型,每天灌胃1次,空白组和模型组予以等量生理盐水灌胃,共28 d.采用小动物超声影像系统检测各组大鼠右下肢膝关节滑膜增生程度评分与增生面积;酶联免疫吸附试验检测各组大鼠血清中白细胞介素-6(IL-6)、IL-17、肿瘤坏死因子-α(TNF-α)水平;苏木精-伊红染色法观察大鼠关节滑膜病理改变;免疫组织化学法检测大鼠关节滑膜组织血小板-内皮细胞黏附分子31(CD31)与血管内皮细胞生长因子受体2(VEGFR2)表达水平.结果:与空白组比较,模型组CIA大鼠右膝关节滑膜区域面积和滑膜增生评分显著增加(P＜0.01);大鼠关节滑膜组织也出现显著病理性改变;血清炎症介质水平升高,同时CD31、VEGFR2表达也显著升高(P＜0.01).与模型组比较,金藤清痹颗粒各剂量组均能明显减小CIA大鼠滑膜增生面积(P＜0.01),金藤清痹颗粒高剂量组大鼠超声滑膜评分显著降低(P＜0.01);金藤清痹颗粒中、高剂量可明显改善大鼠关节滑膜组织病理变化,显著降低IL-6、IL-17、TNF-α水平(P＜0.01),关节滑膜组织CD31、VEGFR2表达也出现一定程度下降(P＜0.01).结论:金藤清痹颗粒能够通过改善CIA大鼠关节滑膜血管新生抑制类风湿关节炎病理进展,达到减轻关节损伤的作用,其机制可能与抑制炎症介质分泌,降低CD31、VEGFR2表达有关.
Objective:To observe the therapeutic effect of Jinteng Qingbi Granule on synovial angiogenesis in collagen-induced ar-thritis(CIA)rats and explore its mechanism.Methods:Sixty SD rats were randomly divided into blank group,model group,metho-trexate group,and Jinteng Qingbi Granule low-,medium-and high-dose groups.The CIA rat model was established by immune in-duction,once a day.The rats in blank group andmodel group were given the same amount of normal saline by gavage once a day for 28 days.Small animal ultrasound imaging was used to detect the synovial hyperplasia score and hyperplasia area in the knee joint of the right lower limb of rats in each group.The serum IL-6,IL-17 and TNF-α were detected by ELISA.HE staining was used to ob-serve the pathological changes of synovium in rats.The expressions of CD31 and VEGFR2 in synovial tissue of rats were determined by IHC.Results:Compared with the conditions in blank group,the synovial area and synovial hyperplasia score of CIA rats in mod-el group were increased(P＜0.01);significant pathological changes were also observed in the synovium of rat joints;serum in-flammatory factors were up-regulated,and CD31 and VEGFR2 were also increased(P＜0.01).Compared with model group,Jinteng Qingbi Granule groups had reduced synovial hyperplasia area in CIA rats(P＜0.01);Jinteng Qingbi Granule high-dose group had decreased synovial score(P＜0.01);Jinteng Qingbi Granule medium-and high-dose groups improved the pathological changes of synovial tissue of rats,reduced IL-6,IL-17 and TNF-α(P＜0.01),and lowered CD31 and VEGFR2 in synovial tissue of joints(P＜0.01).Conclusion:Jinteng Qingbi Granule can inhibit the pathological progress of rheumatoid arthritis by improving the syn-ovial angiogenesis of CIA rats,thereby reducing the joint damage.The mechanism may be related to inhibiting the secretion of in-flammatory factors and down-regulating CD31 and VEGFR2.

Rheumatoid arthritisJinteng Qingbi GranuleSynovitisAngiogenesisTraditional Chinese medicine(TCM)thera-pyCollagen-induced arthritis(CIA)SD ratsPathological mechanismPharmacology

杨家熙、苗宇、万盈盈、孙文婷、王文乾、王皓男、叶婉婷、任建勋、程国良、房定亚、寇秋爱

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中国中医科学院西苑医院,北京,100091

鲁南制药集团股份有限公司,临沂,276005

北京中医药大学,北京,100029

类风湿关节炎 金藤清痹颗粒 滑膜炎 血管新生 中医治疗 胶原诱导性类风湿关节炎 SD大鼠 病理机制 药理作用

中国中医科学院优势病种医院制剂新药研发专项

ZZ15-XY-PT-07

2023

10.3969/j.issn.1673-7202.2023.22.006

世界中医药
世界中医药学会联合会

世界中医药

CSTPCDCHSSCD北大核心
影响因子：1.266
ISSN：1673-7202
年,卷(期)：2023.18(22)
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