Objective:The mechanism of Astragalus on hepatocellular carcinoma was explored from the perspectives of cell experi-ment and network pharmacology.Methods:Relevant gene targets and active components of Astragalus and hepatocellular carcinoma were searched through CNKI,PubMed,TCMSP,GeneCards,and OMIM.The networks of protein-protein interaction(PPI)and As-tragalus-hepatocellular carcinoma interaction were constructed by the STRING database and Cytoscape software.KEGG and GO en-richment analysis was performed with the DAVID database,and molecular docking was performed with the CB-DOCK platform.Cell experiments were performed by MTT assay and Western blot assay to detect cell proliferation and protein expression.Results:Nineteen active ingredients and 250 targets of Astragalus were screened out.GO and KEGG enrichment analysis obtained 549 en-tries and 145 signaling pathways,mainly involved in regulating DNA transcription,cell proliferation,and apoptosis.The pathway of action mainly involved tumor-related pathways,such as cancer pathway,lipid and atherosclerosis,Kaposi's sarcoma infection,and PI3K-AKT signaling pathway.Molecular docking results showed that formononetin,isoflavones,and astragaloside IV,the core com-ponents of for anti-hepatocellular carcinoma of Astragalus,had good binding ability to proteins in the PI3K/AKT signaling pathway.Based on the results of network pharmacology analysis,further cell experiments confirmed that water extract of Astragalus can effec-tively inhibit the proliferation of HepG2 cells and down-regulate the protein expression levels of AKT1,PI3K p1 10,and p-AKT1.Conclusion:Astragalus can inhibit the PI3 K/AKT signaling pathway,exert an inhibitory effect on HepG2 cells,and provide theo-retical support for its application in the clinical treatment of hepatocellular carcinoma.
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