内异止痛方诊治子宫内膜异位症及Wnt/β-联蛋白信号通路调控研究

Diagnosis and Treatment of Endometriosis with Neiyi Zhitong Decoction and Regulation of Wnt/β-catenin Signaling Pathway

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中文摘要：目的:明确内异止痛方对子宫内膜异位症(EMs)的诊疗效果及客观评价其作用机制.方法:采用自体移植法构建EMs模型,将40只造模成功的EMs大鼠按随机数字表法分为4组,另设假手术组8只.并分别给予不同浓度的中药985.3 mg/(kg·d)和245.8 mg/(kg·d)灌胃,西药采用达那唑7.2 mg/(kg·d)灌胃,模型组及假手术组给予生理盐水2 mL/d灌胃,用药4周后观察大鼠异位病灶生长情况,并测量治疗前后体积大小;采用酶联免疫吸附试验法及蛋白质印迹法对各指标进行检测;结果:各观察组异位病灶体积明显缩小(P＜0.01),模型组无明显变化(P＞0.05);与模型组比较,在血清中内异止痛方组可明显降低基质金属蛋白酶-9(MMP-9)、升高组织基质金属蛋白酶抑制物-1(TIMP-1)及E-钙黏着蛋白(E-cadherin)的表达水平,且中药高剂量组优于中药低剂量组(P＜0.05);在组织匀浆中内异止痛方组能降低MMP-9和血管内皮生长因子(VEGF)、β-联蛋白(β-catenin)、升高E-cadherin的表达水平,且中药高剂量组优于低剂量组(P＜0.05).结论:内异止痛方具有抑制异位内膜生长、缩小异位病灶体积作用,同时通过调控Wnt/β-catenin信号通路的关键调节蛋白和下游靶基因使通路处于关闭状态,阻断EMs的发生及进展.

外文摘要：Objective:To determine the effect of Neiyi Zhitong Decoction on the diagnosis and treatment of endometriosis(EMvs)and objectively evaluate its mechanism.Methods:The EMS model was established by autotransplantation,and 40 EM rats were di-vided into four groups according to the random number table method.A sham operation group was set up with eight rats.Different concentrations of traditional Chinese medicine 985.3 mg/(kg·d)and 245.8 mg/(kg·d)were administered by gavage,and dan-azol 7.2 mg/(kg·d)was administered by gavage.The model group and sham operation group were given normal saline of 2 mL/d by gavage.After four weeks of treatment,the growth of ectopic lesions was observed,and the volume of ectopic lesions before and after treatment was measured.The indexes were detected by ELISA and Western Blotting.Results:The volume of ectopic lesions in each treatment group decreased significantly(P＜0.01),but there was no significant change in the model group(P＞0.05).Com-pared with the model group,the expression levels of MMP-9,TIMP-1,and E-cadherin in the serum of the Neiyi Zhitong Decoction group were significantly decreased,and the high-dose group was better than the low-dose group(P＜0.05).In the tissue homoge-nate,the expression levels of MMP-9,VEGF,β-catenin,and E-cadherin in the Neiyi Zhitong Decoction group were decreased,and the high-dose group was better than the low-dose group(P＜0.05).Conclusion:Neiyi Zhitong Decoction can effectively inhibit the growth of ectopic endometrium,reduce the volume of ectopic lesions,shut down the Wnt/β-catenin signaling pathway by regulating the key regulatory proteins and downstream target genes,and block the occurrence and progression of EMs.

外文关键词：

EndometriosisAdhesionWnt/β-catenin signaling pathwayNeiyi Zhitong Decoctionβ-cateninE-cadherinMechanism

作者：

朱小琳、胡晓萌、杨东霞、宋思钰、朱泽文、张诗笛、韩延华、韩亚光

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作者单位：

黑龙江中医药大学附属第二医院,哈尔滨,150001

黑龙江中医药大学,哈尔滨,150040

黑龙江中医药大学附属第一医院,哈尔滨,150040

关键词：

子宫内膜异位症黏附 Wnt/β-catenin信号通路内异止痛方 β-联蛋白 E-钙黏着蛋白作用机制

基金：

国家自然科学基金黑龙江省博士后科研启动金资助项目国家中医药管理局全国中医学术流派传承工作室第二轮建设项目韩延华全国名老中医药专家传承工作室建设项目第六批全国老中医药专家学术经验继承项目

项目编号：

81574015LBH-Q20057国中医药人教函[2019]62号国中医药人教函[2019]41号国中医药人教函[2017]29号

出版年：

2023

DOI：

10.3969/j.issn.1673-7202.2023.23.006

世界中医药

世界中医药学会联合会

世界中医药

CSTPCDCHSSCD北大核心

影响因子：1.266

ISSN：1673-7202

年,卷(期)：2023.18(23)

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