Objective:Aiming at the problem that"nine"in"nine steamings and nine sun-dryings"is not clear,this paper explored the change rule in extracts and polysaccharides in the one to nine steamings of wine-processed Polygonati Rhizoma,and investigated the immune regulation effect of wine-processed Polygonati Rhizoma with different steaming times on immunosuppressed mice,to op-timize the optimal steaming times.Methods:Taking appearance traits,extract content and polysaccharides as the evaluation inde-xes,the comprehensive scoring method was employed to select the top three decoction pieces of wine-processed Polygonati Rhizo-ma.Cyclophosphamide was used to establish a mouse immunosuppression model.The body weight change and immune organ index of the mice after administration of the three batches of wine-processed Polygonati Rhizoma were observed,and the function of phag-ocytic cells was analyzed by the clearance of carbon particles,to obtain the optimal steaming times of wine-processed Polygonati Rhizoma.Results:The comprehensive scores of four steamings and four sun-dryings,five steamings and five sun-dryings as well as six steamings and six sun-dryings of wine-processed Polygonati Rhizoma were the highest,which were 0.72,0.66 and 0.75,respec-tively.Compared with the model group,the body weight change in the groups of four steamings and four sun-dryings,five steamings and five sun-dryings and six steamings and six sun-dryings was increased(P＜0.01,0.05),and the thymus and spleen indices were elevated(P＜0.01).The function of phagocytic cells in the three groups was also improved(P＜0.01,0.05).There was no significant difference in the body weight change and thymus index between the three batches of wine-processed Polygonati Rhizoma,but the spleen index in the group of six steamings and six sun-dryings was higher than that in the group of four steamings and four sun-dryings(P＜0.05),and the correction index in the group of six steamings and six sun-dryings was higher(P＜0.05).Conclu-sion:The comprehensive scores of wine-processed Polygonati Rhizoma after six steamings and six sun-dryings are the highest in terms of appearance traits,extract and polysaccharides.According to the traditional process of four,five and six steamings,wine-pro-cessed Polygonati Rhizoma can regulate immunity,among which,six steamings and six sun-dryings has the most significant effect.Therefore,six steamings is the optimal for wine-processed Polygonati Rhizoma.It is advisable to make yellow essence with six steamings and six sun-dryings.
维普
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