艾灸对溃疡性结肠炎大鼠结肠脂多糖/Toll样受体4信号通路的调节作用

Moxibustion Regulates LPS/TLR4 Signaling Pathway in Colonic Tissue of Rat Model of Ulcerative Colitis

扫码查看

原文链接

万方数据

中文摘要：目的:观察艾灸对溃疡性结肠炎(UC)大鼠结肠黏膜脂多糖(LPS)/Toll样受体4(TLR4)信号通路的调节作用,探讨艾灸干预UC的作用机制.方法:将36只雄性斯泼累格·多雷(SD)大鼠随机分为正常组、UC模型组、艾灸组和西药组,每组8只(剩余4只用于模型鉴定).采用4％浓度的葡聚糖硫酸钠(DSS)溶液自由饮用7 d以建立UC大鼠模型.艾灸组取双侧"天枢穴"和"上巨虚穴"进行隔药饼灸,西药组采用美沙拉嗪肠溶片溶液灌胃.采用酶联免疫吸附测定(ELISA)法检测大鼠血清LPS的蛋白含量;采用免疫组织化学法检测大鼠结肠组织脂多糖结合蛋白(LBP)、分化抗原14(CD14)、TLR4、髓样分化因子88(MyD88)、β干扰素TIR结构域衔接蛋白(TRIF)、白细胞介素6(IL-6)和肿瘤坏死因子-α(TNF-α)的蛋白表达;采用逆转录PCR(RT-PCR)技术检测大鼠结肠组织中IL-6、TNF-α的信使核糖核酸(mRNA)表达.结果:与正常组相比,UC大鼠外周血清LPS的含量、结肠组织LBP、CD14、TLR4、MyD88、TRIF、IL-6和TNF-α的蛋白表达以及IL-6和TNF-α的mRNA表达均显著升高(P＜0.01或P＜0.05),艾灸可降低UC大鼠外周血清LPS的含量、结肠组织LBP、CD14、TLR4、MyD88、TRIF、IL-6和TNF-α的蛋白表达以及IL-6和TNF-α的mRNA表达(P＜0.01或P＜0.05).结论:艾灸可能通过调节LPS/TLR4的信号转导通路来减轻UC大鼠的肠道炎症反应.

外文摘要：Objective:To observe the regulatory effect of moxibustion on the lipopolysaccharide(LPS)/Toll-like receptor 4(TLR4)signaling pathway in the colonic mucosa of the rat model of ulcerative colitis(UC)and explore the mechanism of moxibustion in treating UC.Methods:Thirty-six male SD rats were randomly assigned into normal,UC model,moxibustion,and Western medicine groups.The UC rat model was established by free access to 4％dextran sulphate sodium(DSS)solution for 7 days.Herbs-partition moxibustion was applied on bilateral Tianshu(ST25)and Shangjuxu(ST37)in the moxibustion group,and the solution of mesala-zine enteric-coated tablets was used for gavage in the Western medicine group.Enzyme-linked immunosorbent assay(ELISA)was employed to measure the content of LPS in the serum.Immunohistochemistry was used to detect the protein expression of LPS-bind-ing protein(LBP),cluster of differentiation 14(CD14),TLR4,myeloid differentiation factor 88(MyD88),TIR-domain-containing adapter-inducing interferon-β(TRIF),interleukin(IL)-6,and tumor necrosis factor-α(TNF-α)in the colonic tissue.RT-PCR was employed to determine the mRNA levels of IL-6 and TNF-α in the colonic tissue.Results:Compared with the normal group,the modeling of UC increased content of LPS and up-regulated the protein levels of LBP,CD14,TLR4,MyD88,TRIF,IL-6,and TNF-αand the mRNA levels of IL-6 and TNF-α(P＜0.01 or P＜0.05),which,however,all reduced after moxibustion(P＜0.01 or P＜0.05).Conclusion:Moxibustion may alleviate intestinal inflammation in UC rats by regulating the LPS/TLR4 signaling pathway.

外文关键词：

Ulcerative colitisMoxibustionLipopolysaccharideToll-like receptor 4Signaling pathwayRatColonic mucosaMechanism

作者：

黎梅、王村、吴焕淦、吕思颐、祁琴、李峰、刘慧荣、王晓梅、吴璐一

展开 >

作者单位：

上海中医药大学附属岳阳临床医学院,上海,200437

上海中医药大学,上海,201203

上海市针灸经络研究所,上海,200030

上海市气功研究所,上海,200030

展开 >

关键词：

溃疡性结肠炎艾灸脂多糖 Toll样受体4 信号通路大鼠结肠黏膜机制

基金：

国家自然科学基金国家自然科学基金上海市自然科学基金面上项目上海中医药事业发展三年行动计划项目

项目编号：

814737588177440520ZR1453100ZY2018-2020-CCCX-2004-01

出版年：

2023

DOI：

10.3969/j.issn.1673-7202.2023.24.008

世界中医药

世界中医药学会联合会

世界中医药

CSTPCDCHSSCD北大核心

影响因子：1.266

ISSN：1673-7202

年,卷(期)：2023.18(24)

参考文献量4