Effect of Gentiopicroside on Fructose-Induced Hyperuricemia in Mice
Objective:To investigate the effect of gentiopicroside on fructose-induced hyperuricemia in mice.Methods:The hype-ruricmia KM mice were induced by feeding water containing 10％fructose for consecutive 12 weeks.During the period from 9th week to 12th week,mice from allopurinol group and gentiopicroside groups(80,40,20 mg/kg)received allopurinol and gentiopicro-side,respectively by intragastric administration once a day for 4 weeks.The blood was collected after the last administration,to de-tect glutamic pyruvic transaminase(GPT),glutamic oxaloacetic transaminase(GOT),uric acid(UA),creatinine(Cr)and blood u-rea nitrogen(BUN)in serum.H&E staining was performed to observe the changes in liver and kidney tissues.Superoxide dismutase(SOD),malondialdehyde(MDA),tumor necrosis factor-α(TNF-α)and interleukin 6(IL-6)in kidney tissues were measured by enzyme-linked immunosorbent assay(ELISA),and the activity and expression of XOD in liver were tested by colorimetry and West-ern Blotting.Results:Compared with the model group,the mice in gentiopicroside groups showed a decrease in serum GPT,GOT,UA,Cr and BUN(P＜0.01),with improvement in pathological changes of liver and kidney.The SOD in kidney were increased,whereas MDA,TNF-α and IL-6 were lowered(P＜0.01).Moreover,gentiopicroside reduced the activity and protein expression of XOD in liver(P＜0.01 or P＜0.05).Conclusion:Gentiopicroside down-regulates UA in the fructose-induced hyperuricemia mice by modulating the activity and the expression of XOD,thereby alleviating oxidative stress,and inflammation and protecting the kid-ney.
万方数据
维普
