Determination of Four Chemical Components in Dracocephalum heterophyllum Benth Using Quantitative Analysis of Multi-components by Single Marker
Objective:Simultaneous determination of four chemical constituents in Dracocephalum heterophyllum Benth using quan-titative analysis of multi-components by single marker(QAMS).Methods:Chlorogenic acid(CA),Luteoloside(L1),Luteolin(L2)were used as index components to establish the relative correction factor(fs/i)bby high-performance liquid chromatography(HPLC).The stability and durability of the relative correction factors of the four components were investigated on different chroma-tographic columns and chromatograph.The results of QAMS and external standard method(ESM)were compared.Results:The fs/i of the four components are fCA/RA=1.148、fL1/RA=1.004、fL2/RA=0.689.The rang of fs/i,The RSD on three brands of column and HPLC instruments were 2.95％to 3.61％,The RSD in different column temperatures were 0.90 to 1.28％,The RSD at different flow rates(0.9,1.0,1.1 mL/min)were 1.24 to 2.21％,The results show that the durability of the relative correction factor is good.ESM method and QAMS method were used to determine the contents of four chemical Components in 12 batches of Draco-cephalum heterophyllum Benth and the results showes that there was no significant difference.Conclusion:The determination of four chemical components in Dracocephalum heterophyllum Benth by QAMS is accurate and reliable,which can provide reference for im-proving the quality of Dracocephalum heterophyllum Benth.
万方数据
维普
