Effect of Panax japonicum Saponin on Lung Cancer Rats by Regulating Nuclear Factor-κB Signaling Pathway
Objective:To investigate the effect of Panax japonicus saponin on brain nerve injury,micro vascular density(MVD)and TNF-1β in lung cancer rats by regulation of NF-κB signaling pathway.Methods:Forty SPF male SD rats were randomly divided in-to normal group(A),model group(B),Atractylodes atractylodes polysaccharide(C)group and P.japonicus saponin(D)group,with 10 in each group.Lung cancer modeling was performed in groups B,C and D by endobronchial perfusion of carcinogenic iodide oil.After successful modelling,group C was intragastrically administered with 80 mg/kg A.atractylodes polysaccharide;group D was in-tragastrically administered with 40 mg/kg P.japonicus saponin;groups A and B were given the same volume of normal saline by ga-vage.HE staining was used to observe the pathological morphology of lung tissue,and TUNEL assay was performed to detect brain nerve injury.ELISA was performed to detect the serum TNF-β of rats.MVD in lung tissue was determined by immunohistochemis-try,and NF-κB protein expression in lung tissue was measured by Western blot.Results:Lung tissue and alveoli were normal and intact in group A,while obvious pathological changes were seen in group B.Compared with the condition in group B,the pathologi-cal structure in groups C and D was significantly improved,and group D was better than group C.Compared with group A,group B had increased hippocampal cell apoptosis,MVD,and NF-κB protein expression(P<0.05),and decreased serum TNF-β(P<0.05).Hippocampal cell apoptosis,MVD,NF-κB protein expression and tumor volume in groups C and D were lower than those in group B(P<0.05),while the serum TNF-β was higher(P<0.05),with group D having more obvious changes than group C(P<0.05).Conclusion:P.japonicus saponins may significantly improve brain nerve injury and MVD by negatively regulating NF-κB signaling pathway and increasing TNF-β in rats.