首页|竹节参皂苷调控核因子κB信号通路对肺癌大鼠的影响

竹节参皂苷调控核因子κB信号通路对肺癌大鼠的影响

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目的:探究竹节参皂苷调控核因子κB(NF-κB)信号通路对肺癌大鼠脑神经损伤、微血管密度(MVD)及肿瘤坏死因子-β(TNF-β)表达的影响.方法:选取40只无特定病原体(SPF)级斯泼累格·多雷(SD)雄性大鼠,随机分为正常组、模型组、白术多糖组、竹节参皂苷组,每组10只,对模型组、白术多糖组、竹节参皂苷组采用肺叶支气管内灌注致癌碘化油进行肺癌建模,建模成功后,对白术多糖组灌胃80 mg/kg的白术多糖,对竹节参皂苷组灌胃40 mg/kg的竹节参皂苷,正常组不建模,正常组、模型组同期给予灌胃同体积生理盐水.采用苏木精-伊红(HE)染色法检测肺组织病理形态,TUNEL法检测大鼠脑神经损伤,ELISA法检测大鼠血清中TNF-β水平,免疫组织化学法检测肺组织MVD,蛋白质免疫印迹法检测大鼠肺组织核因子κB蛋白表达.结果:正常组肺组织结构、肺泡正常完整,模型组可见明显病理改变,与模型组比较,白术多糖组、竹节参皂苷组病理结构明显改善,且竹节参皂苷组比白术多糖组改善明显;与正常组比较,模型组海马组织细胞凋亡、MVD、核因子κB蛋白表达明显增多(P<0.05),血清中TNF-β表达明显降低(P<0.05),与模型组比较,白术多糖组、竹节参皂苷组大鼠海马组织细胞凋亡、MVD、核因子κB蛋白表达、肿瘤体积明显降低(P<0.05),血清中TNF-β表达明显升高(P<0.05),且竹节参皂苷组比白术多糖组变化显著(P<0.05).结论:竹节参皂苷可能是通过负向调控NF-κB信号通路显著改善大鼠脑神经损伤及MVD,提高TNF-β表达水平.
Effect of Panax japonicum Saponin on Lung Cancer Rats by Regulating Nuclear Factor-κB Signaling Pathway
Objective:To investigate the effect of Panax japonicus saponin on brain nerve injury,micro vascular density(MVD)and TNF-1β in lung cancer rats by regulation of NF-κB signaling pathway.Methods:Forty SPF male SD rats were randomly divided in-to normal group(A),model group(B),Atractylodes atractylodes polysaccharide(C)group and P.japonicus saponin(D)group,with 10 in each group.Lung cancer modeling was performed in groups B,C and D by endobronchial perfusion of carcinogenic iodide oil.After successful modelling,group C was intragastrically administered with 80 mg/kg A.atractylodes polysaccharide;group D was in-tragastrically administered with 40 mg/kg P.japonicus saponin;groups A and B were given the same volume of normal saline by ga-vage.HE staining was used to observe the pathological morphology of lung tissue,and TUNEL assay was performed to detect brain nerve injury.ELISA was performed to detect the serum TNF-β of rats.MVD in lung tissue was determined by immunohistochemis-try,and NF-κB protein expression in lung tissue was measured by Western blot.Results:Lung tissue and alveoli were normal and intact in group A,while obvious pathological changes were seen in group B.Compared with the condition in group B,the pathologi-cal structure in groups C and D was significantly improved,and group D was better than group C.Compared with group A,group B had increased hippocampal cell apoptosis,MVD,and NF-κB protein expression(P<0.05),and decreased serum TNF-β(P<0.05).Hippocampal cell apoptosis,MVD,NF-κB protein expression and tumor volume in groups C and D were lower than those in group B(P<0.05),while the serum TNF-β was higher(P<0.05),with group D having more obvious changes than group C(P<0.05).Conclusion:P.japonicus saponins may significantly improve brain nerve injury and MVD by negatively regulating NF-κB signaling pathway and increasing TNF-β in rats.

Panax japonicus saponinsNF-κBSignaling pathwayLung cancerRatsBrain nerve injuryMicrovascular densi-tyTNF-β

朱丽、郑丽文、王广梅、李运曼、蔡宝昌、李文婷、毛旭华

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南京市中西医结合医院,南京,210014

南京中医药大学,南京,210023

竹节参皂苷 核因子κB 信号通路 肺癌 大鼠 脑神经损伤 微血管密度 肿瘤坏死因子-β

国家自然科学基金江苏省科技成果转化专项江苏省自然科学基金

81902148BA2018002ZKX17047

2024

世界中医药
世界中医药学会联合会

世界中医药

CSTPCDCHSSCD北大核心
影响因子:1.266
ISSN:1673-7202
年,卷(期):2024.19(2)
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