热痹方对痛风性关节炎小鼠炎症标志物表达的影响

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中文摘要：目的:研究热痹方对痛风性关节炎小鼠的治疗作用及对炎症介质白细胞介素-1β(IL-1β)、IL-6及肿瘤坏死因子-α(TNF-α)的抑制作用.方法:30只C57BL/6小鼠随机分为对照组、模型组、低剂量热痹方组、高剂量热痹方组、秋水仙碱组.除对照组外,其余4组小鼠均在足底关节附近注射3％尿酸钠晶体悬液制备痛风性关节炎(GA)小鼠模型.造模后6 h,对照组和模型组小鼠灌胃给予纯净水,低、高剂量热痹方组小鼠分别灌胃给予热痹方提取物(17.82 g/kg,35.64 g/kg),秋水仙碱组小鼠腹腔注射给予秋水仙碱溶液(0.5 mg/kg).观察治疗前后小鼠体质量、足肿胀、热痛阈的变化;苏木精-伊红染色评估足组织、肝脏和肾脏炎症变化;采用酶联免疫吸附试验(ELISA)法检测足组织中IL-1β、IL-6、TNF-α的含量;实时PCR(RT-PCR)检测足组织中IL-1β、IL-6、TNF-α的基因表达水平.结果:与模型组比较,高剂量热痹方组或秋水仙碱组能显著减轻GA小鼠后爪肿胀程度(P＜0.01),提高热痛耐受性(P＜0.001),减少关节组织的炎症细胞浸润,且明显降低关节组织炎症介质IL-1β、IL-6、TNF-α的蛋白和mRNA水平(P＜0.001).另外,高剂量热痹方和秋水仙碱对GA小鼠治疗效果无明显差异(P＞0.05).结论:热痹方能有效改善GA小鼠症状并抑制关节炎症反应,且对肝肾功能无明显影响.

外文标题：Effects of Rebi Formula on the Expression of Inflammatory Markers in Gouty Arthritis Mice

外文摘要：Objective:To investigate the therapeutic effect of Rebi formula on mice with gouty arthritis(GA)and the inhibition effects on inflammation factors interleukin-1β(IL-1 β),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α).Methods:Thirty C57BL/6 mice were randomly divided into control group,model group,low-dose Rebi formula group,high-dose Rebi formula group and colchicine group.Except for the control group,mice in the other four groups were injected with 3％monosodium urate crystal suspension near the plantar joint to generate mouse model of GA.Six hours after modeling,mice in the control group and the model group were given distilled water by intragastric administration.The mice in the low-dose and high-dose Rebi formula groups were given Rebi formula extracts(17.82 g/kg,35.64 g/kg)by gavage,and mice in the colchicine group were given colchicine solution intraperitoneally(0.5 mg/kg).The changes in body weight,foot swelling and thermal pain threshold were observed before and after the treatment.Hematoxylin-eosin staining was used to evaluate the changes of inflammation in foot tissue,liver and kidney.The con-tents of IL-1 β,IL-6 and TNF-α in foot tissue were determined by ELISA.Gene expression levels of IL-1 β,IL-6 and TNF-α in foot tissues were detected by qRT-PCR.Results:Compared with model group,high-dose Rebi formula or colchicine treatment signifi-cantly reduced the swelling degree of the hind paw of GA mice(P＜0.01),improved the heat pain tolerance(P＜0.001),and re-duced the inflammatory cell infiltration of joint tissue.The protein and mRNA expression levels of inflammatory factors IL-1 β,IL-6 and TNF-α were significantly decreased in joint tissue(P＜0.001).In addition,there was no significant difference in the therapeu-tic effect between high-dose Rebi formula group and colchicine group(P＞0.05).Conclusion:Rebi formula can effectively allevi-ate the symptoms and inhibit joint inflammation in mice with GA,with no significant effect on liver and kidney function.

外文关键词：

Traditional Chinese medicineRebi formulaGouty arthritisMouseInflammatory cytokinesInterleukin-1 βInter-leukin-6Tumor necrosis factor-α

作者：

王沁筠、王晓赟、袁璐莹、徐浩、施杞、梁倩倩、李宁、王拥军

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作者单位：

上海中医药大学附属龙华医院,上海,200032

上海中医药大学脊柱病研究所,上海,200032

上海中医药大学康复医学院,上海,201203

上海市针灸经络研究所,上海,200030

上海筋骨理论与治法教育部重点实验室,上海,200120

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关键词：

中医药热痹方痛风性关节炎小鼠炎症介质白细胞介素-1β 白细胞介素-6 肿瘤坏死因子-α

基金：

教育部创新团队项目上海市卫生健康委中医药科研项目创新团队类项目国家中医药局青年岐黄学者项目上海市重中之重研究中心项目中国博士后科学基金面上项目

项目编号：

IRT12702022CX0012015RA40022022ZZ010092022M722163

出版年：

2024

DOI：

10.3969/j.issn.1673-7202.2024.02.006

世界中医药

世界中医药学会联合会

世界中医药

CSTPCDCHSSCD北大核心

影响因子：1.266

ISSN：1673-7202

年,卷(期)：2024.19(2)

参考文献量24