Objective:To study the effect of Shuji Anshen Decoction on neurotransmitters and anxiety in p-chlorophenylalanine(PCPA)-induced insomnia rats.Methods:The rats were divided into control group,model group,low-dose Chinese medicine group(8.5 g/kg),medium-dose Chinese medicine group(13 g/kg),high-dose Chinese medicine group(17 g/kg)and western medicine group(0.26 mg/kg).After modeling,the rats in each group were continuously administered for 7 days.Hematoxylin-eosin(HE)staining was used to observe the pathological changes of hippocampus.Enzyme-linked immunosorbent assay(ELISA)was used to detect the contents of 5-hydroxyindoleacetic acid(5-HIAA),serotonin(5-HT),norepinephrine(NE),dopamine(DA)and melato-nin(MT)in hippocampus.Western blotting was used to detect the protein expression of acetylcholinesterase(AChE)and brain-de-rived neurotrophic factor(BDNF)in hippocampus.Results:The rat model of insomnia was successfully established.Shuji Anshen Decoction could increase the body weight and sleep time,increase the number of pyramidal cells in CA1 region of hippocampus,in-crease the contents of 5-HIAA,5-HT,AChE,BDNF in hippocampus and MT in serum,and reduce the contents of NE and DA in hippocampus.Conclusion:Shuji Anshen Decoction could improve neurotransmitter and anxiety in PCPA-induced insomnia rats,and the mechanism was related to the increase of 5-HT content.In addition,Shuji Anshen Decoction also improved the learning and memory ability of PCPA-induced insomnia rats by activating the protein expression of BDNF,a marker protein of synaptic plasticity in the prefrontal cortex.
关键词
枢机安神汤/失眠/神经递质/焦虑/乙酰胆碱酯酶/脑源性神经营养因子/褪黑素/学习记忆能力
Key words
Shuji Anshen Decoction/Insomnia/Neurotransmitter/Anxiety/Acetylcholinesterase/Brain-derived neurotrophic fac-tor/Melatonin/Learning and memory ability
维普
万方数据