Objective:To investigate the effect of formononetin on the proliferation and apoptosis of glioma cells and its molecular mechanism.Methods:A subcutaneous xenograft tumor mouse model was established,and mice were orally administered with form-ononetin at 5.72,14.31,22.9,and 31.49 mg/kg.Tumor weight and volume changes were observed,and the expression of prolifera-ting cell nuclear antigen(PCNA)in tumor tissues was detected using immunohistochemistry.TJ905 cells were treated with different concentrations(20,50,80,and 110 μmol/L)of formononetin,and the cells were divided into blank control group,110 μmol/L for-mononetin group,110 µmol/L formononetin+miR-1229 group,110 μmol/L formononetin+miR-1229+ZDHHC9 group,miR-NC group,and miR-1229 mimic group.Cell proliferation ability was detected using Edu assay and CCK-8 assay.Cell apoptosis was de-tected using TUNEL assay.The expression levels of miR-1229 and ZDHHC9 mRNA were measured using real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)assay.The targeting binding of miR-1229 to ZDHHC9 was verified using online websites and dual luciferase reporter gene assay.Results:Compared with the blank control group,the tumor volume,weight,and PCNA protein expression in the formononetin-treated groups at different doses were significantly reduced(all P＜0.05).Compared with the control group,treatment with different concentrations(20,50,80,and 110 μmol/L)of formononetin significantly reduced cell proliferation rate and increased apoptosis rate(all P＜0.05).Compared with the 110 µmol/L formononetin group,the cell pro-liferation rate and viability in the 110 µmol/L formononetin+miR-1229 group were significantly reduced,and the apoptosis rate was significantly increased(all P＜0.05).Compared with the 110 μmol/L formononetin+miR-1229 group,the cell proliferation rate and viability in the 110 µmol/L formononetin+miR-1229+ZDHHC9 group were significantly increased,and the apoptosis rate was significantly decreased(all P＜0.05).The dual luciferase reporter gene assay confirmed the targeted binding of miR-1229 to ZDHHC9.Conclusion:Formononetin may inhibit the proliferation of glioma cells and induce their apoptosis through the miR-1229/ZDHHC9 molecular axis.
维普
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