Effects of Guashi Decoction Combined with Danggui Shaoyao Powder on PCOS-IR Mice
Objective:To explore the effects of Guashi Decoction combined with Danggui Shaoyao Powder on sex hormones and en-dometrial inflammatory factors in mice models with polycystic ovary syndrome combined with insulin resistance(PCOS-IR).Meth-ods:PCOS-IR mice models were constructed with letrozole and a high-fat diet,and the success of the model was evaluated by ob-serving changes in the estrous cycle and measuring fasting blood glucose and fasting insulin in mice.The mice were randomly divid-ed into a control group,model group,and traditional Chinese medicine(TCM)group,and the TCM was given Guashi Decoction combined with Danggui Shaoyao Powder by gavage after successful modeling,while the control group and model group were given an equal volume of pure water.Serum follicle-stimulating hormone(FSH),luteinizing hormone(LH),and total testosterone(T)levels were measured after 21 days of intervention by enzyme-linked immunosorbent assay(ELISA)method,and the levels of tumor necro-sis factor-α(TNF-α),interleukin-1β(IL-1β),and interleukin-6(IL-6)in endothelial tissue were measured.Results:After model-ing,the mice in the control group had a normal estrous cycle,while the mice in the model group had a disturbed estrous cycle,most-ly in the interestrous phase.The homeostasis model assessment-insulin resistance(HOMA-IR)of the control mice was less than 2.69,while that in the model group was greater than 2.69.After 21 days of the administration,the serum LH,T,and LH/FSH of mice in the TCM group were lower than those in the model group(P＜0.05),and the mRNA and protein expression levels of TNF-α,IL-1β,αnd IL-6 in endothelial tissue were lower than those in the model group(P＜0.05).Conclusion:Guashi Decoction com-bined with Danggui Shaoyao Powder can improve the serum hormone level,reduce the level of endothelial inflammatory factors,and improve the endothelial inflammatory status in PCOS-IR mice.
万方数据
维普
