目的:基于缺血缺氧脑瘫大鼠神经功能评分(Zea-Longa评分)、脑组织肉眼观和大脑海马区胱天蛋白酶-9(Caspase-9)、胱天蛋白酶-3(Caspase-3)的表达水平变化,探讨缺血缺氧模型脑瘫大鼠的有效时长.方法:选取3周龄斯泼累格·多雷(SD)健康大鼠,随机分为正常组和模型组,采用改良的Rice-Vannucci方法建立脑瘫模型,造模后第1、7、14、21天,观察各组大鼠的一般情况并进行神经功能评分,在第7、14、21天分批处死大鼠并取脑组织,观察各组大鼠左侧脑组织,检测海马区Caspase-9、Caspase-3的表达水平.结果:一般情况:造模后第1天,与正常组比较,模型组大鼠左侧瞳孔缩小、姿势异常、自发或夹尾左旋、自主活动减少、兴奋性降低、肌肉颤动、头颤,抽搐,抓取时抵抗反应明显,随着时间延长,以上异常行为逐渐消失,造模后21 d基本消失不见,但左侧瞳孔一直小于对侧;Zea-Longa评分:与正常组比较,模型组造模后7、14 d Zea-Longa评分较高,差异有统计学意义(P＜0.05);脑组织肉眼观:与正常组比较,模型组造模后7、14及21 d大鼠左侧脑组织有不同程度的萎缩和坏死;免疫组化结果:与正常组比较,模型组造模后7 d、14 d Caspase-9、Caspase-3的表达水平均显著升高,差异有统计学意义(P＜0.05).结论:3周龄缺血缺氧脑瘫模型大鼠的有效时长为14～21 d,可干预14 d.
Time Effect of Cerebral Palsy in Rat Model of Ischemia and Hypoxia
Objective:To explore the time effect of cerebral palsy in the rat model of ischemia and hypoxia based on the Zea-Longa score,appearance of the brain tissue,and the expression levels of caspase-9 and caspase-3 in the hippocampus.Methods:Healthy SD rats of 3 weeks old were selected and randomized into normal and model groups.The rat model of cerebral palsy was established by the modified Rice-Vannucci method.On days 1,7,14,and 21 after modeling,the general conditions of the rats in each group was observed and the Zea-Longa score was recorded.On days 7,14,and 21,the modeled rats were sacrificed in batches and the brain tissue was collected.The left brain was observed,and the expression levels of caspase-9 and caspase-3 in the hippocampus were de-termined.Results:Compared with the normal group,the rats modeled for 1 day presented contraction of the left pupil,abnormal pos-tures,spontaneous or nip-tail left-turning,reduced spontaneous activity,decreased excitability,muscle fibrillation,head fibrillation,twitch,and obvious resistance to capture.The abnormalities gradually weakened over time and almost disappeared 21 days after modeling,with the left pupil being always smaller than the right pupil.The model group had higher Zea-Longa score than the normal group on days 7 and 14(P＜0.05).Compared with the normal group,the model group showed different degrees of atrophy and nec-rosis in the left brain tissue 7,14,and 21 days after modeling.Furthermore,the model group had higher expression levels of caspase-9 and caspase-3 than the normal group on days 7 and 14(P＜0.05).Conclusion:The modeling of cerebral palsy was ef-fective for 14 to 21 days in the rat model(3 weeks old)of ischemic and hypoxia,which indicated an intervention periods of 14 days.
Model of ischemia and hypoxiaRat model of cerebral palsyEffective durationGeneral conditionsZea-Longa scoreAppearance of the brain tissueCaspase-9Caspase-3
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