Exploring the of Effects of Different Doses of Elemene Emulsion Injection on Virus in Rat Feces Based on Metagenomic Sequencing Technology
Objective:To investigate the effects of different doses of elemene emulsion injection on virus in the feces of rats.Meth-ods:Eighteen specific pathogen-free healthy male Sprague-Dawley(SD)rats were randomly divided into three groups:low-dose el-emene emulsion injection group[L-EEI,20 mg/(kg·d)],high-dose elemene emulsion injection group[H-EEI,40 mg/(kg·d)]and Control group(equal volume of normal saline),with six rats in each group.After 14 days of continuous administration,fresh feces were collected for metagenomic sequencing,followed by bioinformatic analysis of the detected viruses.Results:Venn di-agram analysis showed that the number of unique viruses in control,L-EEI and H-EEI groups were 6,6 and 10,respectively,with 77 common viruses identified at the species level across the three groups.PCA analysis showed that the data of the L-EEI group were significantly different from those of the other groups(P=0.036 at the genus level,P=0.012 at the species level).NMDS a-nalysis showed that the degree of data aggregation in L-EEI and H-EEI groups was higher(genus level:Stress=0.093,species lev-el:Stress=0.129).A total of 10 viruses showed significant differences among the three groups(P＜0.01).The effects of different doses of EEI on viral abundance could be divided into three categories:1)viral abundance was positively or negatively correlated with EEI dose;2)Compared with the control group,L-EEI increased virus abundance,but H-EEI had no significant effect on virus abundance;3)Compared with the control group,L-EEI caused a decrease in virus abundance,but H-EEI had no significant effect on virus abundance.Conclusion:Different doses of EEI could induce changes in the composition and structure of the virus in the feces of rats,and different viruses show varying sensitivities to different doses of EEI.
MetagenomeVirusElemeneTumorMicrobiotaTraditional Chinese MedicineBioinformation AnalysisGut Mi-crobiota
万方数据
维普
